(5 references)-
- 抗原 See all APOB 抗体
- APOB (Apolipoprotein B (APOB))
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This APOB antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)
- 交叉反应 （详细）
- Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with human apoLipoprotein B and has negligible cross-reactivity with Type A-I, A-II, C-I, C-II, C-III, E and J apoLipoproteins. Specific cross reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible.
- 纯化方法
- This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities.
- 免疫原
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Immunogen: apoLipoprotein Type B was isolated from human plasma by density gradient centrifugation followed by HPLC purification.
Immunogen Type: Native Protein
- 亚型
- IgG
- Top Product
- Discover our top product APOB Primary Antibody
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anti-Apolipoprotein B (APOB) (AA 2747-2913) antibody
APOB 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Apolipoprotein B (APOB) (AA 2747-2913) antibodyAPOB 适用: 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C3 unconjugated
anti-Apolipoprotein B (APOB) (AA 28-330) antibodyAPOB 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Apolipoprotein B (APOB) antibodyAPOB 适用: 人 WB, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 6G6 unconjugated
anti-Apolipoprotein B (APOB) (AA 3365-3548) antibodyAPOB 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Apolipoprotein B (APOB) (AA 28-127) antibodyAPOB 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3E5 unconjugated
anti-Apolipoprotein B (APOB) (AA 4031-4080) antibodyAPOB 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Apolipoprotein B (APOB) (AA 1501-1700) antibodyAPOB 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Apolipoprotein B (APOB) antibodyAPOB 适用: 人, 小鼠, 大鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-Apolipoprotein B (APOB) antibodyAPOB 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunohistochemistry Dilution: 1:50 - 1:500
Application Note: Anti-apoLipoprotein antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis.
Western Blot Dilution: 1:200 - 1:1,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:2,000 - 1:10,000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
Stabilizer: None
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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: "A critical role of VMP1 in lipoprotein secretion." in: eLife, Vol. 8, (2020) (PubMed).
: "Nuclear lipid droplets derive from a lipoprotein precursor and regulate phosphatidylcholine synthesis." in: Nature communications, Vol. 10, Issue 1, pp. 473, (2019) (PubMed).
: "Spontaneous severe hypercholesterolemia and atherosclerosis lesions in rabbits with deficiency of low-density lipoprotein receptor (LDLR) on exon 7." in: EBioMedicine, Vol. 36, pp. 29-38, (2019) (PubMed).
: "Chylomicrons produced by Caco-2 cells contained ApoB-48 with diameter of 80-200 nm." in: Physiological reports, Vol. 2, Issue 6, (2014) (PubMed).
: "The association of hepatitis C virus glycoproteins with apolipoproteins E and B early in assembly is conserved in lipoviral particles." in: The Journal of biological chemistry, Vol. 289, Issue 27, pp. 18904-13, (2014) (PubMed).
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: "A critical role of VMP1 in lipoprotein secretion." in: eLife, Vol. 8, (2020) (PubMed).
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- 抗原
- APOB (Apolipoprotein B (APOB))
- 别名
- APOLIPOPROTEIN B (APOB 产品)
- 别名
- FLDB antibody, LDLCQ4 antibody, Ac1-060 antibody, Apo B-100 antibody, ApoB-100 antibody, ApoB-48 antibody, AI315052 antibody, apob-100 antibody, apob-48 antibody, APOB-100 antibody, ApoB(100) antibody, APOB antibody, apolipoprotein B antibody, APOB antibody, Apob antibody
- 背景
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Synonyms: APOB protein antibody, Apolipoprotein B 100 antibody, Apolipoprotein B 48 antibody, Apolipoprotein B antibody, FLDB antibody
Background: Anti Apolipoprotein B antibody recognizes the gene product of APOB that is the primary apolipoprotein of low-density lipoproteins, which is responsible for carrying cholesterol to tissues. While it is unclear exactly what functional role APOB plays in LDL, it is the primary apolipoprotein component and is absolutely required for its formation. What is clear is that the APOB on the LDL particle acts as a ligand for LDL receptors in various cells throughout the body. Through a mechanism that is not fully understood, high levels of APOB can lead to plaques that cause vascular disease (atherosclerosis), leading to heart disease. There is considerable evidence that levels of APOB are a better indicator of heart disease and cardiovascular risk than total cholesterol or LDL. However, primarily for historic reasons, cholesterol, and more specifically, LDL-cholesterol, remains the primary lipid test for the risk factor of atherosclerosis.
Gene Name: APOB
- 基因ID
- 338
- UniProt
- Q7Z7Q0
- 途径
- Lipid Metabolism
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