Urm1 抗体
Quick Overview for Urm1 抗体 (ABIN116960)
抗原
See all Urm1 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This product is prepared from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated below. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum.
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纯化方法
- Multi-step process.
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免疫原
- Recombinant yeast Urm1 protein.
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anti-Ubiquitin Related Modifier 1 (Urm1) (AA 1-63) antibody
Urm1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Related Modifier 1 (Urm1) antibodyUrm1 适用: 大鼠, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Related Modifier 1 (Urm1) (Internal Region) antibodyUrm1 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Related Modifier 1 (Urm1) antibodyUrm1 适用: Saccharomyces cerevisiae WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Suitable for ELISA (1/2,000-1/10,000) and Immunoblotting (1/1,000). Although not tested,this antibody is likely functional in Immunohistochemistry and Immunoprecipitation. This antibody using the specified conditions may recognize other prominent intrinsicbands (UBLs or their conjugates).
Other intrinsic bands are readily detectable in yeast lysates at lower antibody dilutions. A 12 kDa band corresponding to yeast Urm1 is detected. Most yeast cell lysates can beused as a positive control without induction or stimulation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with 0.1 mL of deionized water (or equivalent).
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浓度
- 5.0 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 0.01 % (w/v) Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing. Dilute only prior to immediate use
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储存条件
- 4 °C/-20 °C
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储存方法
- Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
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- Urm1 (Ubiquitin Related Modifier 1 (Urm1))
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别名
- URM1
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背景
- Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12. Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not conjugated to other proteins. Urm1 is a newly identified ubiquitin related modifier. Urm 1 is a 99-amino acid protein terminated with glycine-glycine. Target proteins are conjugated to Urm1 via its C-terminal glycine. Initially Urm1 forms a thioester with a novel E1-like protein, Uba4.Synonyms: C9orf74, URM-1, Ubiquitin-related modifier 1, YIL008W
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基因ID
- 854809
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NCBI登录号
- NP_012258
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UniProt
- P40554
抗原
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