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- 抗原 See all NEDD8 抗体
- NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))
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适用
- Saccharomyces cerevisiae
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This NEDD8 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- 特异性
- This purified polyclonal antibody reacts with yeast Rub1. This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
- 纯化方法
- Multi-step process.
- 免疫原
- This purified antibody was prepared from rabbit serum after repeated immunizations with full-length recombinant yeast Rub1 protein.
- Top Product
- Discover our top product NEDD8 Primary Antibody
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anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) antibody
NEDD8 适用: 人 WB, IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 1A7 unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) antibodyNEDD8 适用: 人 WB, ELISA, IF, IHC (p), FACS 宿主: 小鼠 Monoclonal 1A7 unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) antibodyNEDD8 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) (N-Term) antibodyNEDD8 适用: 人, 小鼠, 大鼠, 猴, Cow, 非洲爪蟾 WB, IHC, IF, IHC (p), FACS, IP 宿主: 兔 Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) antibodyNEDD8 适用: 人 WB, IF, IHC (p), FACS, EIA 宿主: 小鼠 Monoclonal 1A7 unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) (AA 1-81) antibodyNEDD8 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) (N-Term) antibodyNEDD8 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) antibodyNEDD8 适用: Saccharomyces cerevisiae WB 宿主: 兔 Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) (AA 1-32) antibodyNEDD8 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) (N-Term) antibodyNEDD8 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Suitable for Immunoblotting (Western Blot: 1/500-1/2,000) and ELISA (1/1,000-1/5,000). Although not tested, this antibody is likely functional in Immunohistochemistry andImmunoprecipitation. A 6 kDa band corresponding to yeast Rub1 is detected. Most yeast cell lysates can be used as a positive control without induction or stimulation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Restore with 0.5 mL of deionized water (or equivalent).
- 浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing. Dilute only prior to immediate use
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
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- 抗原
- NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))
- 别名
- RUB1 (NEDD8 产品)
- 别名
- NEDD-8 antibody, Rub1 antibody, CG10679 antibody, Dmel\\CG10679 antibody, NEDD8 antibody, dNEDD8 antibody, nedd8 antibody, ubiquitin antibody, si:zc14a17.5 antibody, GB17785 antibody, DDBDRAFT_0218116 antibody, DDBDRAFT_0238041 antibody, DDB_0218116 antibody, DDB_0238041 antibody, nedd8.S antibody, neural precursor cell expressed, developmentally down-regulated 8 antibody, neural precursor cell expressed, developmentally down-regulated gene 8 antibody, CG10679 gene product from transcript CG10679-RB antibody, Nedd8 antibody, ubiquitin-like protein Nedd8 antibody, neural precursor cell expressed, developmentally down-regulated 8 L homeolog antibody, NEDD8 antibody, Nedd8 antibody, nedd8 antibody, nedd8.L antibody
- 背景
- Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg12, Hub1. Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not proteolytically processed or conjugated to other proteins. Rub1 and the corresponding human homolog Nedd8 are activated by the E1 ubiquitin-activating enzyme UBA2, that forms isopeptide linkages between thio esters. Nedd8 shows 80 % homology to ubiquitin. The best known targets of Rub1 modification are members of the cullin family. Cullins are subunits of an E3-ubiquitin ligase complex called the Skp1-/Cul1/Cdc53-F-box (SCF). The SCF promotes transfer of ubiquitin from a ubiquitin conjugating enzyme (E2) to the target protein. Rub1 modification may regulate SCF function or localization.Synonyms: NEDD8-like protein RUB1, Ubiquitin-like protein RUB1, YDR139C
- 基因ID
- 851717
- NCBI登录号
- NP_010423
- UniProt
- Q03919
- 途径
- Ubiquitin Proteasome Pathway
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