我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

ATG12 抗体

Name: ATG12 抗体
SKU: ABIN116953
Availability: OutOfStock

ATG12 适用: Saccharomyces cerevisiae WB, EIA 宿主: 兔 Polyclonal unconjugated

Immunoblot of APG12 fusion protein. (ATG12 抗体)

Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs). (ATG12 抗体)

2 images

产品编号 ABIN116953

发货至: 中国

Datasheet as PDF

抗原 See all ATG12 抗体

ATG12 (Autophagy Related 12 (ATG12))
适用
77

36

26

6

6

6

6

5

4

4

4

4

2

2

2

2

2

2

2

2

1
Saccharomyces cerevisiae
宿主
74

19

2
兔
Compatible Secondaries
克隆类型
74

21
多克隆
标记
47

7

6

6

6

4

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This ATG12 antibody is un-conjugated
应用范围
73

40

30

28

21

13

13

10

8

3

3

1

1

1

1
Western Blotting (WB), Enzyme Immunoassay (EIA)

特异性

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

交叉反应（详细）

Species reactivity (tested):S. cerevisiae, Yeast

纯化方法

A multi-step process which includes Delipidation, Salt Fractionation and Ion exchange chromatography followed by extensive dialysis against the buffer stated below.

免疫原

This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast APG12 protein.

Top Product

Discover our top product ATG12 Primary Antibody
anti-Autophagy Related 12 (ATG12) (N-Term) antibody
ATG12 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 猴, 小鸡, 兔, Pig, Cat, 驴, 山羊, Hamster, 绵羊, Avian WB, IF 宿主: 山羊 Polyclonal unconjugated

anti-Autophagy Related 12 (ATG12) antibody
ATG12 适用: 人 WB, ELISA, IHC, IF 宿主: 小鼠 Monoclonal 43CT73-3-5-5-4 unconjugated

anti-Autophagy Related 12 (ATG12) (AA 101-187) antibody
ATG12 适用: 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Autophagy Related 12 (ATG12) (AA 1-74) antibody
ATG12 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-Autophagy Related 12 (ATG12) (AA 1-30), (N-Term) antibody
ATG12 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB7475 unconjugated

anti-Autophagy Related 12 (ATG12) (AA 1-50) antibody
ATG12 适用: 人, 小鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Autophagy Related 12 (ATG12) antibody
ATG12 适用: 人 WB, IHC, IP 宿主: 兔 Monoclonal unconjugated

anti-Autophagy Related 12 (ATG12) (AA 1-140) antibody
ATG12 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1F9 unconjugated

anti-Autophagy Related 12 (ATG12) (AA 1-74) antibody
ATG12 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2H8 unconjugated

anti-Autophagy Related 12 (ATG12) (AA 79-128) antibody
ATG12 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 斑马鱼, 猴, 小鸡, 兔, Pig, Bat, 非洲爪蟾 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

应用备注

This purified polyclonal antibody reacts with yeast APG12 by Western blot and ELISA. This antibody using the specified conditions may recognize other prominent intrinsicbands (UBLs or conjugates).
Other intrinsic bands are readily detectable at lower dilutions. A 22.1 kDa band corresponding to yeast APG12 is detected. Most yeast cell lysates can be used as a positive control without induction or stimulation. Recommended Dilutions: ELISA: 1/1,000-1/5,000. Western Blot: 1/200. Although not tested, this antibody is likely functional in Immunohistochemistry andImmunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

限制

仅限研究用
溶解方式

Restore with 0.1 mL of deionized water or equivalent.

浓度

5.0 mg/mL (by UV absorbance at 280 nm)

缓冲液

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing. Dilute only prior to immediate use

储存条件

4 °C/-20 °C

储存方法

Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
抗原

ATG12 (Autophagy Related 12 (ATG12))

别名

ATG12 / APG12 (ATG12 产品)

背景

Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12. Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not conjugated to other proteins. In yeast, autophagy, the delivery of cytoplasmic components to the lysosome/vacuole for degradation, requires a ubiquitin-like protein conjugation system, in which Apg12 is covalently bound to Apg12-Apg5 and Apg16.Synonyms: APG12-like, APG12L, Autophagy-related protein 12, Ubiquitin-like protein ATG12

基因ID

852518

NCBI登录号

NP_009776

UniProt

P38316

途径

Autophagy