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FLIP 抗体 (AA 1-480)

Name: FLIP 抗体 (AA 1-480)
Brand: antibodies-online
SKU: ABIN1169019
Availability: OutOfStock

This anti-FLIP antibody is a 大鼠单克隆 antibody detecting FLIP in WB 和 IP. Suitable for 人和小鼠. This Primary Antibody has been cited in 7+ publications.

Detection of human and mouse FLIP in 293T cells transfected with a human (lane 2) or mouse FLIP L (lane 3) expression plasmid using anti-FLIP, mAb (Dave-2) . (FLIP 抗体 (AA 1-480))

1 image

(7 references)

产品编号 ABIN1169019

发货至: 中国

Datasheet as PDF

Quick Overview for FLIP 抗体 (AA 1-480) (ABIN1169019)

抗原

See all FLIP (CFLAR) 抗体

FLIP (CFLAR) (CASP8 and FADD-Like Apoptosis Regulator (CFLAR))

适用

人, 小鼠

宿主

大鼠

克隆类型

单克隆

标记

This FLIP antibody is un-conjugated

应用范围

Western Blotting (WB), Immunoprecipitation (IP)

克隆位点

Dave-2

Discover our top product CFLAR Primary Antibody

抗原表位
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AA 1-480

特异性

Recognizes an epitope (aa 1-200) present in both short (FLIPS) and long (FLIPL) splice variants of human and mouse FLIP.

交叉反应

人, 小鼠

免疫原

Recombinant human FLIP (aa 1-480).

亚型

IgG2a
anti-CASP8 and FADD-Like Apoptosis Regulator (CFLAR) (AA 100-251) antibody
CFLAR 适用: 人, 小鼠 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 7A3D12 unconjugated

anti-CASP8 and FADD-Like Apoptosis Regulator (CFLAR) (AA 178-277) antibody
CFLAR 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 2F12 unconjugated

anti-CASP8 and FADD-Like Apoptosis Regulator (CFLAR) (AA 100-251) antibody
CFLAR 适用: 人, 小鼠 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal unconjugated

anti-CASP8 and FADD-Like Apoptosis Regulator (CFLAR) (AA 1-250) antibody
CFLAR 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-CASP8 and FADD-Like Apoptosis Regulator (CFLAR) (AA 191-209) antibody
CFLAR 适用: 人, 小鼠 WB, ELISA, IHC, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-CASP8 and FADD-Like Apoptosis Regulator (CFLAR) (AA 178-277) antibody
CFLAR 适用: 人 WB, ELISA, IF, IP 宿主: 小鼠 Monoclonal 1G1 unconjugated

anti-CASP8 and FADD-Like Apoptosis Regulator (CFLAR) (AA 161-480) antibody
CFLAR 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-CASP8 and FADD-Like Apoptosis Regulator (CFLAR) (AA 7-100) antibody
CFLAR 适用: 人, 小鼠, 大鼠 WB, ELISA, IF (cc), FACS, IHC (p), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-CASP8 and FADD-Like Apoptosis Regulator (CFLAR) (AA 401-480) antibody
CFLAR 适用: 人, 小鼠, 大鼠 WB, ELISA, IF (cc), IHC (p), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-CASP8 and FADD-Like Apoptosis Regulator (CFLAR) (N-Term) antibody
CFLAR 适用: 人, 小鼠, 大鼠, Cow, 犬, 马 WB 宿主: 兔 Polyclonal unconjugated

应用备注

Optimal working dilution should be determined by the investigator.

限制

仅限研究用
状态

Liquid

浓度

Lot specific

缓冲液

In PBS containing 10 % glycerol and 0.02 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.

有效期

12 months
Van Opdenbosch, Van Gorp, Verdonckt, Saavedra, de Vasconcelos, Gonçalves, Vande Walle, Demon, Matusiak, Van Hauwermeiren, DHont, Hochepied, Krautwald, Kanneganti, Lamkanfi: "Caspase-1 Engagement and TLR-Induced c-FLIP Expression Suppress ASC/Caspase-8-Dependent Apoptosis by Inflammasome Sensors NLRP1b and NLRC4." in: Cell reports, Vol. 21, Issue 12, pp. 3427-3444, (2018) (PubMed).

Dannappel, Vlantis, Kumari, Polykratis, Kim, Wachsmuth, Eftychi, Lin, Corona, Hermance, Zelic, Kirsch, Basic, Bleich, Kelliher, Pasparakis: "RIPK1 maintains epithelial homeostasis by inhibiting apoptosis and necroptosis." in: Nature, Vol. 513, Issue 7516, pp. 90-4, (2014) (PubMed).

Lens, Kataoka, Fortner, Tinel, Ferrero, MacDonald, Hahne, Beermann, Attinger, Orbea, Budd, Tschopp: "The caspase 8 inhibitor c-FLIP(L) modulates T-cell receptor-induced proliferation but not activation-induced cell death of lymphocytes." in: Molecular and cellular biology, Vol. 22, Issue 15, pp. 5419-33, (2002) (PubMed).

Gómez-Angelats, Cidlowski: "Protein kinase C regulates FADD recruitment and death-inducing signaling complex formation in Fas/CD95-induced apoptosis." in: The Journal of biological chemistry, Vol. 276, Issue 48, pp. 44944-52, (2001) (PubMed).

Micheau, Lens, Gaide, Alevizopoulos, Tschopp: "NF-kappaB signals induce the expression of c-FLIP." in: Molecular and cellular biology, Vol. 21, Issue 16, pp. 5299-305, (2001) (PubMed).

Djerbi, Darreh-Shori, Zhivotovsky, Grandien: "Characterization of the human FLICE-inhibitory protein locus and comparison of the anti-apoptotic activity of four different flip isoforms." in: Scandinavian journal of immunology, Vol. 54, Issue 1-2, pp. 180-9, (2001) (PubMed).

Rescigno, Piguet, Valzasina, Lens, Zubler, French, Kindler, Tschopp, Ricciardi-Castagnoli et al.: "Fas engagement induces the maturation of dendritic cells (DCs), the release of interleukin (IL)-1beta, and the production of interferon gamma in the absence of IL-12 during DC-T cell cognate ..." in: The Journal of experimental medicine, Vol. 192, Issue 11, pp. 1661-8, (2000) (PubMed).
抗原

FLIP (CFLAR) (CASP8 and FADD-Like Apoptosis Regulator (CFLAR))

别名

FLIP

背景

FLIP is an apoptosis regulator protein which functions as a crucial link between cell survival and cell death pathways in mammalian cells and acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. FLIP lacks enzymatic (caspase) activity. FLIP is highly expressed in skeletal muscle, pancreas, heart, kidney, placenta and peripheral blood leukocytes.

UniProt

O15519

途径

Apoptosis, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development