PKM2 抗体
Our Local Distributor
北京 101111
Quick Overview for PKM2 抗体 (ABIN116809)
抗原
See all PKM2 抗体适用
宿主
克隆类型
标记
应用范围
-
-
特异性
- Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Pyruvate Kinase [Rabbit Muscle]. Cross reactivity may occur against other mammalian Pyruvate Kinase, but were not specifically determined.
-
纯化方法
- Prepared from monospecific antiserum by delipidation and defibrination.
-
免疫原
- Pyruvate Kinase from Rabbit Muscle.
-
-
-
-
应用备注
-
Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation. Recommended Dilutions: This product has been assayed against 1.0 g of Pyruvate Kinase[Rabbit Muscle] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purifiedanti-Goat IgG and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as asubstrate for 30 minutes at room temperature. A working dilution of 1: 2,000 to 1: 12,000 ofthe reconstitution concentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
溶解方式
- Restore with 2.0 mL of deionized water (or equivalent).
-
浓度
- 40.0 mg/mL (by Refractometry)
-
缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Avoid repeated freezing and thawing. Dilute only prior to immediate use
-
储存条件
- 4 °C/-20 °C
-
储存方法
- Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
-
-
- PKM2 (Pyruvate Kinase M2 (PKM2))
-
别名
- PKM2
-
背景
- Synonyms: CTHBP, Cytosolic thyroid hormone-binding protein, M2-PK, OIP-3, OIP3, Opa-interacting protein 3, PK2, PK3, PKM, Pyruvate kinase 2/3, Pyruvate kinase M1/M2, Pyruvate kinase isozymes M1/M2, Pyruvate kinase muscle, Pyruvate kinase muscle isozyme, THBP1
-
基因ID
- 100008676
-
NCBI登录号
- NP_001182573
-
UniProt
- P11974
-
途径
- Warburg Effect
抗原
-