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Hexokinase 1 抗体

This 兔 多克隆 anti-Hexokinase 1 antibody specifically detects Hexokinase 1 in WB 和 EIA. The antibody is reactive with Saccharomyces cerevisiae samples.
产品编号 ABIN116752
发货至: 中国
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Quick Overview for Hexokinase 1 抗体 (ABIN116752)

抗原

See all Hexokinase 1 (HK1) 抗体
Hexokinase 1 (HK1)

适用

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Saccharomyces cerevisiae

宿主

  • 110
  • 15

克隆类型

  • 91
  • 34
多克隆

标记

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This Hexokinase 1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Enzyme Immunoassay (EIA)
  • 特异性

    Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Hexokinase [Yeast]. Cross reactivity against Hexokinase from other tissues and species may occur but have not been specifically determined.

    纯化方法

    Prepared from monospecific antiserum by a delipidation and defibrination.

    免疫原

    Hexokinase [Yeast].
  • 应用备注

    Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of Hexokinase[Yeast] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purifiedanti-Rabbit IgG [H&L] (Goat) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonicacid]) as a substrate for 30 minutes at room temperature. A working dilution of 1: 10,000 to1: 40,000 of the reconstitution concentration is suggested for this product.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 状态

    Liquid

    溶解方式

    Restore with 2.0 mL of deionized water (or equivalent).

    浓度

    80.0 mg/mL (by Refractometry)

    缓冲液

    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide as preservative.

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing. Dilute only prior to immediate use

    储存条件

    4 °C/-20 °C

    储存方法

    Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
  • 抗原

    Hexokinase 1 (HK1)

    别名

    Hexokinase-1

    背景

    Synonyms: Brain form hexokinase, HK1, Hexokinase type I

    基因ID

    2542634

    NCBI登录号

    NP_592948

    UniProt

    Q09756

    途径

    Carbohydrate Homeostasis, Warburg Effect
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