Hexokinase 1 抗体
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北京 101111
Quick Overview for Hexokinase 1 抗体 (ABIN116752)
抗原
See all Hexokinase 1 (HK1) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Hexokinase [Yeast]. Cross reactivity against Hexokinase from other tissues and species may occur but have not been specifically determined.
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纯化方法
- Prepared from monospecific antiserum by a delipidation and defibrination.
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免疫原
- Hexokinase [Yeast].
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应用备注
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Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of Hexokinase[Yeast] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purifiedanti-Rabbit IgG [H&L] (Goat) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonicacid]) as a substrate for 30 minutes at room temperature. A working dilution of 1: 10,000 to1: 40,000 of the reconstitution concentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- Restore with 2.0 mL of deionized water (or equivalent).
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浓度
- 80.0 mg/mL (by Refractometry)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing. Dilute only prior to immediate use
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储存条件
- 4 °C/-20 °C
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储存方法
- Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
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- Hexokinase 1 (HK1)
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别名
- Hexokinase-1
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背景
- Synonyms: Brain form hexokinase, HK1, Hexokinase type I
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基因ID
- 2542634
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NCBI登录号
- NP_592948
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UniProt
- Q09756
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途径
- Carbohydrate Homeostasis, Warburg Effect
抗原
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