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GFP 抗体 (TRITC)

GFP 适用: Aequorea victoria WB, IF, EIA 宿主: 山羊 Polyclonal TRITC
产品编号 ABIN116750
发货至: 中国
  • 抗原 See all GFP 抗体
    GFP (Green Fluorescent Protein (GFP))
    适用
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    Aequorea victoria
    宿主
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    山羊
    克隆类型
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    多克隆
    标记
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    This GFP antibody is conjugated to TRITC
    应用范围
    • 172
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    Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)
    特异性
    This antibody detects Fluorescent Protein (GFP). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Peroxidase and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum. No reaction was observed against Human, Mouse and Rat Serum Proteins.
    产品特性
    Absorption / Emission: 550 nm / 570 nm
    Molar Ratio: 2.9 moles TRITC per mole of Goat IgG
    纯化方法
    Immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
    免疫原
    GST-Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria
    Top Product
    Discover our top product GFP Primary Antibody
  • 应用备注
    ELISA (Sandwich or Capture, ELISA for direct binding recognizes wild type, recombinantand enhanced forms of GFP, for sandwich ELISA titrate GFP in solution using either form ofthe antibody as the capture or detection antibodies, detection antibody is typically
    限制
    仅限研究用
  • 溶解方式
    Restore with 1.0 mL of deionized water (or equivalent).
    浓度
    1.0 mg/mL (by UV absorbance at 280 nm)
    缓冲液
    0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0 as a buffer, 10 mg/mL Bovine Serum Albumin (BSA) IgG and (protease free) as stabilizer, 0.01 % (w/v) Sodium azide as preservative
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing. Protect from light.
    储存条件
    4 °C/-20 °C
    储存方法
    Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Niv, Keiner, Krishna, Witte, Lie, Redecker: "Aberrant neurogenesis after stroke: a retroviral cell labeling study." in: Stroke; a journal of cerebral circulation, Vol. 43, Issue 9, pp. 2468-75, (2012) (PubMed).

    Suzuki, Mogami, Ihara, Urano: "Unique secretory dynamics of tissue plasminogen activator and its modulation by plasminogen activator inhibitor-1 in vascular endothelial cells." in: Blood, Vol. 113, Issue 2, pp. 470-8, (2009) (PubMed).

  • 抗原
    GFP (Green Fluorescent Protein (GFP))
    别名
    GFP (GFP 产品)
    别名
    green fluorescent protein antibody, gfp antibody
    物质类
    Viral Protein
    背景
    Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology. YFP differs from GFP due to a mutation at T203Y, antibodies raised against full-length GFP should also detect YFP and other variants.Synonyms: GFP-Tag, Green fluorescent protein
    基因ID
    6100
    UniProt
    P42212
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