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- 抗原 See all GLUD1 抗体
- GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))
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适用
- Cow
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This GLUD1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- 特异性
- Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Glutamate Dehydrogenase [Bovine Liver]. BLAST analysis was used to determine that cross reactivity is suggested for both mitochondrial and brain isoforms (GDH1 and GDH2), from both bovine and human sources. Additionally similar reactivity is suggested for most primate species including green monkey, white gibbon, chimpanzee orangutan, and gorilla. A high degree of sequence homology is also noted for GDH from chicken, mouse, rat, dog, and other mammals as well as Xenopus tropicalis, zebrafish, rainbow trout and Atlantic salmon. Cross reactivity against Glutamate Dehydrogenase from other tissues and species may occur but have not been specifically determined.
- 纯化方法
- Delipidation and defibrination.
- 免疫原
- A full length Glutamate Dehydrogenase protein isolated from Bovine Liver.
- Top Product
- Discover our top product GLUD1 Primary Antibody
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anti-Glutamate Dehydrogenase 1 (GLUD1) antibody
GLUD1 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Dehydrogenase 1 (GLUD1) (AA 54-240) antibodyGLUD1 适用: 人 WB, IHC, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Dehydrogenase 1 (GLUD1) antibodyGLUD1 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Glutamate Dehydrogenase 1 (GLUD1) (N-Term) antibodyGLUD1 适用: 人, 小鼠, 大鼠, Cow, 马, Pig, 斑马鱼, 犬, 兔, 绵羊, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Dehydrogenase 1 (GLUD1) (AA 65-273) antibodyGLUD1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Dehydrogenase 1 (GLUD1) (N-Term) antibodyGLUD1 适用: 人, 小鼠, 大鼠, Cow, 马, Pig, 斑马鱼, 犬, 绵羊, Saccharomyces cerevisiae WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Dehydrogenase 1 (GLUD1) (AA 182-231) antibodyGLUD1 适用: 人, 小鼠, 大鼠, Cow, 马, Pig, 斑马鱼, 兔, 小鸡 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Dehydrogenase 1 (GLUD1) antibodyGLUD1 适用: 人 WB, ELISA, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Dehydrogenase 1 (GLUD1) (AA 93-107) antibodyGLUD1 适用: 人, 小鼠, 马, Pig, 猴 WB, IHC, ELISA, IHC (p) 宿主: 山羊 Polyclonal unconjugated
anti-Glutamate Dehydrogenase 1 (GLUD1) (N-Term) antibodyGLUD1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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This antibody has been tested for use in ELISA and by Western blot. Bovine glutamatedehydrogenase exists as a homohexamer located within the mitochondrial matrix. Expect aband approximately 62 kDa in size corresponding to glutamate dehydrogenase monomersubunit by Western blotting in the appropriate cell or tissue extract. Recommended Dilutions: ELISA: 1/4,000-1/16,000Western blot: 1/1,000-1/3,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
- Restore with 2.0 mL of deionized water (or equivalent).
- 浓度
- 85.0 mg/mL (by refractometry)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store vial at 2-8 ° C prior to restoration. Following restoration product can be stored undiluted at 2-8 ° for up to one month or (in aliquots) at -20 °C or below.
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- 抗原
- GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))
- 别名
- Glutamate Dehydrogenase 1 (GLUD1 产品)
- 背景
- Glutamate is a major excitatory neurotransmitter. One enzyme central to the metabolism of glutamate is glutamate dehydrogenase (GDH1, EC 1.4.1.3), that catalyzes the reversible deamination of L-glutamate to 2-oxoglutarate using NAD+ or NADP+. Mammalian GDH is composed of six identical subunits, and the regulation of GDH is very complex. It has been a major goal to identify the substrate and regulatory binding sites of GDH. It is only in recent years that the three-dimensional structure of GDH from microorganisms is available. Very recently, crystallization of bovine liver GDH was reported for the first time from the mammalian sources. However, remarkably little is known about the detailed structure of mammalian GDH, especially the brain enzymes.Synonyms: GDH1, GLUD, GLUD1
- 基因ID
- 2746
- NCBI登录号
- NP_005262
- UniProt
- P00367
- 途径
- Positive Regulation of Peptide Hormone Secretion, Warburg Effect
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