DNASE1 抗体
Quick Overview for DNASE1 抗体 (ABIN116689)
抗原
See all DNASE1 抗体适用
宿主
克隆类型
标记
应用范围
-
-
特异性
- Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Deoxyribonuclease I [Bovine Pancreas]. Cross reactivity against Deoxyribonuclease I from other tissues and species may occur but have not been specifically determined.
-
纯化方法
- Prepared from monospecific antiserum by a delipidation and defibrination.
-
免疫原
- Deoxyribonuclease I from bovine pancreas.
-
-
anti-Deoxyribonuclease I (DNASE1) (AA 19-259) antibody
DNASE1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Deoxyribonuclease I (DNASE1) (AA 22-282) antibodyDNASE1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Deoxyribonuclease I (DNASE1) antibodyDNASE1 适用: 人 WB, ICC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Deoxyribonuclease I (DNASE1) (AA 1-252) antibodyDNASE1 适用: 人 WB, IHC, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Deoxyribonuclease I (DNASE1) (AA 19-259) antibodyDNASE1 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal 2-1# unconjugated
anti-Deoxyribonuclease I (DNASE1) (AA 78-231) antibodyDNASE1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Deoxyribonuclease I (DNASE1) (AA 87-121) antibodyDNASE1 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB49355 unconjugated
anti-Deoxyribonuclease I (DNASE1) antibodyDNASE1 适用: 大鼠, 小鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-Deoxyribonuclease I (DNASE1) (N-Term) antibodyDNASE1 适用: 人, 大鼠, Cow WB 宿主: 兔 Polyclonal unconjugated
anti-Deoxyribonuclease I (DNASE1) (AA 19-259) antibodyDNASE1 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal 7# unconjugated
-
-
应用备注
- Suitable for Immunoblotting (Western or Dot blot), ELISA and most immunological methodsrequiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg ofDeoxyribonuclease I [Bovine Pancreas] in a standard sandwich ELISA using Peroxidaseconjugated Affinity Purified anti-Rabbit IgG [H&L] code R1364HRP and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 100 to 1: 1,000 of the reconstitution concentration is suggested forthis product.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
溶解方式
- Restore with 2.0 mL of deionized water (or equivalent).
-
缓冲液
- 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4 containing 0.01 % (w/v) Sodium Azide as preservative.
-
储存液
- Sodium azide
-
注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Dilute only prior to immediate use
-
储存条件
- -20 °C
-
储存方法
- Store vial at -20 °C prior to opening. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing.
-
-
- DNASE1 (Deoxyribonuclease I (DNASE1))
-
别名
- Deoxyribonuclease-1
-
背景
- Deoxyribonuclease I (EC 3.1.21.1) gene is approximately 3.2 kb long with 9 exons separated by 8 introns. In the form of a bovine pancreatic enzyme preparation, it occupies an important place in the history of protein chemistry and enzymology: it was the first enzyme to be recognized as specific for DNA, it was the first DNase to be crystallized, and it was the first DNase for which a specific protein inhibitor was characterized. DNase I is a Ca2+ and Mg2+ dependant endonuclease. DNase I is synthesized in the pancreas and stored in zymogen granules. It has been used to reduce the viscosity of cystic fibrosis sputum. A DNase I-like enzyme appears to catalyze the degradation of chromatin to oligo- and mononucleosomes during apoptosis. A recent study has demonstrated an endonuclease with activity and antigenicity indistinguishable from DNase I in thymocytes, cells susceptible to apoptosis. DNase I is an endonuclease that hydrolyzes double-stranded or single stranded DNA preferentially at sites adjacent to pyrimidine nucleotides. The product of hydrolysis is a complex mixture of 5'-phosphate mononucleotides and oligonucleotides. In the presence of Mg ion, DNase I attacks each strand of DNA independently and the cleavage sites are random.Synonyms: DNASE1, DNL1, DNase I, DRNI, Deoxyribonuclease I
-
基因ID
- 1773
-
NCBI登录号
- NP_005214
-
UniProt
- P24855
抗原
-
