Clusterin 抗体
Our Local Distributor
北京 101111
Quick Overview for Clusterin 抗体 (ABIN116548)
抗原
See all Clusterin (CLU) 抗体适用
宿主
克隆类型
标记
应用范围
-
-
特异性
- This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities. Typically less than 1 % cross reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with apoLipoprotein J and has negligible cross-reactivity with Type A-I, A-II, B, C-I, C-II, C-III and E apoLipoproteins. Non-specific cross reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible.
-
纯化方法
- Immunoaffinity chromatography
-
免疫原
- ApoLipoprotein Type J was isolated from human plasma by density gradient centrifugation followed by HPLC purification.
-
-
anti-Clusterin (CLU) (AA 331-449) antibody
CLU 适用: 人, 大鼠, 小鼠 ELISA, IHC (p), FACS, IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Clusterin (CLU) (AA 229-446) antibodyCLU 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Clusterin (CLU) (AA 227-448) antibodyCLU 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Clusterin (CLU) (AA 228-449) antibodyCLU 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Clusterin (CLU) (AA 226-447) antibodyCLU 适用: 兔 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C10 unconjugated
anti-Clusterin (CLU) (C-Term) antibodyVerified CLU 适用: 人 WB, IHC, ELISA, IF 宿主: 山羊 Polyclonal unconjugated
anti-Clusterin (CLU) (AA 23-120) antibodyCLU 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Clusterin (CLU) (AA 71-99), (N-Term) antibodyCLU 适用: 人, 大鼠, 小鼠 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB18701 unconjugated
anti-Clusterin (CLU) (AA 227-447) antibodyCLU 适用: 大鼠 WB, IHC 宿主: 小鼠 Monoclonal C1 unconjugated
anti-Clusterin (CLU) (C-Term) antibodyVerified CLU 适用: 大鼠, 小鼠 WB, ELISA, IF 宿主: 山羊 Polyclonal unconjugated
-
-
应用备注
- Has been used for indirect trapping ELISA for quantitation of antigen in serum using astandard curve, for immunoprecipitation and for western blotting for highly sensitivequalitative analysis. Recommended dilutions: ELISA 1: 5,000 - 1: 10,000. Western blot 1: 5,000 - 1: 10,000.
-
限制
- 仅限研究用
-
-
-
浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
-
缓冲液
- 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0, containing 0.01 % (w/v) Sodium Azide as preservative
-
储存液
- Sodium azide
-
注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Avoid repeated freezing and thawing. Dilute only prior to immediate use.
-
储存条件
- 4 °C/-20 °C
-
储存方法
- This product is stable at 2-8 °C as an undiluted liquid for up to one month. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20 °C or below.
-
-
- Clusterin (CLU)
-
别名
- Apolipoprotein J (Apo J)
-
背景
- At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individual's relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). Apolipoprotein J is the major secreted product of Sertoli cells and is thought to play a critical role in spermatogenesis. The protein was shown to be a normal constituent of human blood and is also thought to be a control mechanism of the complement cascade. It prevents the binding of a C5b-C7 complex to the membrane of the target cell and in this way inhibits complement-mediated cytolysis. Apolipoprotein J is overexpressed in human prostate and breast cancers and in squamous cell carcinomas. Suppression of Apolipoprotein J renders these cells sensitive to chemotherapeutic drug-mediated apoptosis. It has been proposed that elevated levels of Apolipoprotein J may promote oncogenic transformation and tumor progression by interfering with BAX proapoptotic activities.Synonyms: 40, 40, AAG4, APOJ, Aging-associated gene 4 protein, CLI, Clusterin, Complement cytolysis inhibitor, Complement-associated protein SP-40, KUB1, Ku70-binding protein 1, NA1/NA2, SP-40, Testosterone-repressed prostate message 2
-
基因ID
- 1191
-
NCBI登录号
- NP_976084
-
UniProt
- P10909
-
途径
- Apoptosis, Negative Regulation of intrinsic apoptotic Signaling
抗原
-
