APOC3 抗体
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北京 101111
Quick Overview for APOC3 抗体 (ABIN116546)
抗原
See all APOC3 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This product has been prepared by Immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities. Typically less than 1 % cross reactivity against other types of ApoLipoprotein was detected by ELISA against purified standards. This antibody recognizes Human ApoLipoprotein C-III and has negligible cross-reactivity with Type A-I, A-II, B, C-I, C-II, E and J ApoLipoproteins. Non-specific cross reaction of anti-ApoLipoprotein antibodies with other Human serum proteins is negligible.
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纯化方法
- Immunoaffinity Chromatography.
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免疫原
- Synthetic full-length Apolipoprotein Type C-III (not selected epitopes) using conventional peptide technology.
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应用备注
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This Apolipoprotein antibody has been used for indirect trapping ELISA for quantitation ofantigen in serum using a standard curve, for Immunoprecipitation, Western blotting forhighly sensitive qualitative analysis and for Immunohistochemistry. Recommended Dilutions: ELISA: 1/5,000-1/10,000. Western blot: 1/5,000-1/10,000. Immunoprecipitation: 1/100. Immunohistochemistry Paraffin Sections: 2.5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0, with 0.01 % Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing. Dilute only prior to immediate use.
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储存条件
- -20 °C
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储存方法
- Store the antibody undiluted (in aliquots) at -20 °C.
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- APOC3 (Apolipoprotein C-III (APOC3))
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别名
- Apolipoprotein C III (Apo CIII)
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背景
- At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). Apolipoprotein C-III is a very low density lipoprotein (VLDL) protein. It inhibits lipoprotein lipase and hepatic lipase and it is thought to delay catabolism of triglyceride-rich particles. An increase in ap °C-III levels induces the development of hypertriglyceridemia.Synonyms: Apo-CIII, Ap °C-III, Ap °C3, Apolipoprotein C-III, Apolipoprotein C3
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基因ID
- 345
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NCBI登录号
- NP_000031
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UniProt
- P02656
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途径
- Carbohydrate Homeostasis, Lipid Metabolism
抗原
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