APOA1 抗体
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北京 101111
Quick Overview for APOA1 抗体 (ABIN116540)
抗原
See all APOA1 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Typically less than 1 % cross reactivity against other types of Apolipoprotein was detected by ELISA against purified standards. This antibody reacts with Human Apolipoprotein A-I and has negligible cross-reactivity with Type A-II, B, C-I, C-II, C-III, E and J Apolipoproteins. Non-specific cross reaction of anti-Apolipoprotein antibodies with other Human serum proteins is negligible.
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交叉反应 （详细）
- Species reactivity (tested):Human.
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纯化方法
- Immunoaffinity Chromatography using immobilized antigens followed by extensive cross-adsorption against other Apolipoproteins and human serum proteins to remove any unwanted specificities.
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免疫原
- Apolipoprotein Type A-I was isolated from Human plasma by density gradient centrifugation followed by HPLC purification.
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anti-Apolipoprotein A-I (APOA1) antibody
APOA1 适用: 小鼠 WB, IHC, ELISA, IP 宿主: 山羊 Polyclonal unconjugated
anti-Apolipoprotein A-I (APOA1) (AA 122-267) antibodyAPOA1 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal C13 unconjugated
anti-Apolipoprotein A-I (APOA1) (AA 25-264) antibodyAPOA1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Apolipoprotein A-I (APOA1) (AA 25-265) antibodyAPOA1 适用: 兔 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C3 unconjugated
anti-Apolipoprotein A-I (APOA1) (AA 1-267) antibodyAPOA1 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal S1 unconjugated
anti-Apolipoprotein A-I (APOA1) (AA 1-267) antibodyAPOA1 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Apolipoprotein A-I (APOA1) (AA 10-37) antibodyAPOA1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Apolipoprotein A-I (APOA1) (AA 51-150) antibodyAPOA1 适用: 人, 小鼠, 大鼠, 小鸡 WB, ELISA, IHC (p), FACS, IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Apolipoprotein A-I (APOA1) (AA 122-267) antibodyAPOA1 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C10 unconjugated
anti-Apolipoprotein A-I (APOA1) (AA 20-267) antibodyAPOA1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
- Anti-Apolipoprotein antibodies have been used for Indirect trapping ELISA for quantitationof antigen in serum using a standard curve, for Immunoprecipitation and for Westernblotting for highly sensitive qualitative analysis. Recommended dilutions: ELISA: 1/10,000-1/20,000. Western blot: 1/5,000-1/10,000. Immunoprecipitation: 1/100. Immunohistochemistry: 1/50-1/200.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0, 0.01 % Sodium Azide, None
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store vial at -20 °C prior to opening. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20 °C or below.
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: "Molecular characterization of EmABP, an apolipoprotein A-I binding protein secreted by the Echinococcus multilocularis metacestode." in: Infection and immunity, Vol. 77, Issue 12, pp. 5564-71, (2009) (PubMed).
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- APOA1 (Apolipoprotein A-I (APOA1))
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别名
- Apolipoprotein A I (APO AI)
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背景
- Apolipoprotein A I promotes cholesterol efflux from tissues to the liver for excretion. Apolipoprotein A I is the major protein component of high density lipoprotein (HDL) in the plasma. Synthesized in the liver and small intestine, it consists of two identical chains of 77 amino acids, an 18 amino acid signal peptide is removed co-translationally and a 6 amino acid propeptide is cleaved post-translationally. Apolipoprotein A I is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. Defects in the Apolipoprotein A I gene are associated with HDL deficiency and Tangier disease. The therapeutic potential of apoA-I has been recently assessed in patients with acute coronary syndromes, using a recombinant form of a naturally occurring variant of apoA-I. The availability of recombinant normal apoA-I should facilitate further investigation into the potential usefulness of apoA-I in preventing atherosclerotic vascular diseases.Synonyms: APOA1, Apo-AI, ApoA-I, ApoAI
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基因ID
- 335
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NCBI登录号
- NP_000030
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UniProt
- P02647
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途径
- Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Lipid Metabolism
抗原
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