LGALS1/Galectin 1 抗体
Quick Overview for LGALS1/Galectin 1 抗体 (ABIN116481)
抗原
See all LGALS1/Galectin 1 (LGALS1) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Recognizes Galectin-1
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交叉反应 (详细)
- Species reactivity (tested):Human.
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纯化方法
- Affinity Chromatography employing immobilized Human Galectin-1 matrix.
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免疫原
- E. Coli derived Recombinant Human Galectin-1.
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应用备注
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Sandwich ELISA: To detect Human Galectin-1 (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Human Galectin-1 (PP1223B1 and PP1223B2)as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinantHuman Galectin-1. Western Blot: To detect Human Galectin-1 by this analysis, the antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hGalectin-1 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2 without preservatives.
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))
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别名
- Galectin-1
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背景
- The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin 1 may act as an autocrine negative growth factor that regulates cell proliferation. It also may regulate cell apoptosis and cell differentiation. It binds beta-galactoside, CD45, CD3 and CD4. Galectin 1 inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. It is found in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney, placenta and hematopoietic cells.Synonyms: 14 kDa lectin, Beta-galactoside-binding lectin L-14-I, Galaptin, Galectin1, HBL, HPL, LGALS1, Lactose-binding lectin 1, Lectin galactoside-binding soluble 1, MAPK-activating protein PM12, S-Lac lectin 1
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分子量
- approx. 150 kDa
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基因ID
- 3956
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NCBI登录号
- NP_002296
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UniProt
- P09382
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途径
- Carbohydrate Homeostasis
抗原
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