CXCL12 抗体 (Biotin)
Quick Overview for CXCL12 抗体 (Biotin) (ABIN116455)
抗原
See all CXCL12 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Reacts with Mouse Stromal Cell Derived Factor-1 alpha (SDF-1 alpha). Other species not tested.
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纯化方法
- Affinity Chromatography.
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免疫原
- Highly pure (>98%) recombinant Mouse SDF-1 alpha. Remarks: Molecular Weight: ~150 kDa
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anti-Chemokine (C-X-C Motif) Ligand 12 (CXCL12) antibody
CXCL12 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Ligand 12 (CXCL12) (AA 23-89) antibodyCXCL12 适用: 人 WB, IHC, ICC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Ligand 12 (CXCL12) (AA 31-80) antibodyCXCL12 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Ligand 12 (CXCL12) (AA 22-89) antibodyCXCL12 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Ligand 12 (CXCL12) (C-Term) antibodyCXCL12 适用: 人, 小鼠, 犬, 小鸡, 非洲爪蟾, Bat, 豚鼠, 马, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Ligand 12 (CXCL12) (C-Term) antibodyCXCL12 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Ligand 12 (CXCL12) antibodyCXCL12 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Ligand 12 (CXCL12) (AA 24-70) antibodyCXCL12 适用: 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Ligand 12 (CXCL12) (C-Term) antibodyCXCL12 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Ligand 12 (CXCL12) (AA 23-89) antibodyCXCL12 适用: 人 WB 宿主: 小鼠 Monoclonal C3 unconjugated
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应用备注
- Direct ELISA: To detect Mouse SDF-1 alpha by direct ELISA (using 100 μL/well antibodysolution) this antibody can be used at a concentration of 0.15-0.30 μg/mL. Used inconjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant Mouse SDF-1 alpha. Sandwich ELISA: To detect Mouse SDF-1 alpha by sandwich ELISA (using 100 μL/wellantibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. Thisbiotinylated polyclonal antibody, in conjunction with Anti-Mouse SDF-1 alpha (PP1217P1 orPP1217P2) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well ofrecombinant MOuse SDF-1 alpha. Western Blot: To detect Mouse SDF-1alpha by Western Blot analysis this antibody can beused at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant Mouse SDF-1 alpha is 1.5-3.0 ng/lane, undereither reducing or non-reducing conditions.
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限制
- 仅限研究用
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溶解方式
- Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2 without preservatives
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))
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别名
- CXCL12 / SDF1
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背景
- SDF1 alpha and 1 beta are small cytokines that belong to the intercrine family, members of which activate leukocytes and are often induced by proinflammatory stimuli such as lipopolysaccharide, TNF or IL1. SDF1 alpha has been shown to be chemotactic for lymphocytes and is recently reported to be a ligand for CXCR4 (LESTR/fusin), a co-receptor for HIV1 entry into T cells. SDF-1 binding to CXCR4 inhibits HIV1 entry.Synonyms: C-X-C motif chemokine 12, IRH, Intercrine reduced in hepatomas, PBSF, Pre-B cell growth-stimulating factor, SDF1A, SDF1B, Stromal cell-derived factor 1, hIRH
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分子量
- approx. 150 kDa
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基因ID
- 20315
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NCBI登录号
- NP_001012495
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UniProt
- P40224
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途径
- Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling
抗原
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