KIT Ligand 抗体
Quick Overview for KIT Ligand 抗体 (ABIN116453)
抗原
See all KIT Ligand (KITLG) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Recognizes Rat SCF
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纯化方法
- Affinity Chromatography employing an immobilized Rat SCF matrix.
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免疫原
- Highly pure (>98%) recombinant Rat SCF.
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应用备注
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Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of ratSCF(15 ng/mL), a concentration of 0.08-0.12 μg/mL of this antibody is required. ELISA: To detect rat SCF by direct ELISA (using 100l/well antibody solution) aconcentration of at least 0.5g/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of0.2-0.4 ng/well of recombinant rat SCF. Western Blot: To detect rat SCF by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant rat SCF is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2 without preservatives.
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- KIT Ligand (KITLG)
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别名
- KITLG / SCF
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背景
- Synonyms: KITL, Kit ligand, MGF, Mast cell growth factor, Stem cell factor, c-Kit ligand
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基因ID
- 60427
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NCBI登录号
- NP_068615
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UniProt
- P21581
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途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
抗原
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