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Midkine 抗体 (Biotin)

This 兔 多克隆 antibody specifically detects Midkine in WB 和 EIA. It exhibits reactivity toward 人.
产品编号 ABIN116440
发货至: 中国
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Quick Overview for Midkine 抗体 (Biotin) (ABIN116440)

抗原

See all Midkine (MDK) 抗体
Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))

适用

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宿主

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克隆类型

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多克隆

标记

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This Midkine antibody is conjugated to Biotin

应用范围

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Western Blotting (WB), Enzyme Immunoassay (EIA)
  • 纯化方法

    Affinity chromatography.

    免疫原

    Highly pure (>98%) recombinant hMidkine.
  • 应用备注

    ELISA: To detect hMidkine by direct ELISA (using 100 l/well antibody solution) thisantibody can be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction withcompatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinanthMidkine. Western Blot: To detect hMidkine by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hMidkine is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 溶解方式

    Restore to a concentration of 50 μg/mL with sterile PBS solution containing 0.1 % BSA.

    缓冲液

    PBS, pH 7.2 without preservatives.

    储存液

    Without preservative

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
  • 抗原

    Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))

    别名

    Midkine

    背景

    Midkine exhibits neurite outgrowth-promoting activity and may play a role in nervous system development and/or maintenance. Its expression is predominant only for a short period from approximately one-half to two-thirds of the way through gestation, before and after that, it is barely detectable. Midkine was first found in differentiating mouse teratocarcinoma cells. It has neurotrophic activities and is mitogenic to certain, but not to all, fibroblast cell lines.Synonyms: Amphiregulin-associated protein, MDK, MK, MK1, Midgestation and kidney protein, NEGF2, Neurite outgrowth-promoting factor 2, Neurite outgrowth-promoting protein

    基因ID

    4192

    NCBI登录号

    NP_001012333

    UniProt

    P21741

    途径

    RTK signaling, M Phase, Skeletal Muscle Fiber Development
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