TNFSF14 抗体
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北京 101111
Quick Overview for TNFSF14 抗体 (ABIN116433)
抗原
See all TNFSF14 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Affinity chromatography employing immobilized hLIGHT matrix.
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免疫原
- Highly pure (>98%) recombinant hLIGHT
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anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14) (AA 62-240) antibody
TNFSF14 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14) (AA 61-170) antibodyTNFSF14 适用: 人 WB, ELISA, IP, IHC (p) 宿主: 小鼠 Monoclonal 4E3 unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14) (AA 141-240) antibodyTNFSF14 适用: 小鼠, 大鼠 WB, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14) (AA 51-239) antibodyTNFSF14 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14) (AA 62-240) antibodyTNFSF14 适用: 人 WB 宿主: 小鼠 Monoclonal C1 unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14) (AA 1-239) antibodyTNFSF14 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14) (Internal Region) antibodyVerified TNFSF14 适用: 人 WB, ELISA, IF 宿主: 山羊 Polyclonal unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14) (AA 58-239) antibodyTNFSF14 适用: 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14) (Internal Region) antibodyTNFSF14 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Neutralization. ELISA: To detect hLIGHT by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2 - 0.4 ng/well of recombinant hLIGHT. Western Blot: To detect hLIGHT by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hLIGHT is 1.5 -3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore in sterile water to a concentration of > 0.2 mg/mL.
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缓冲液
- 0.5 X PBS pH 7.4 without preservatives.
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- TNFSF14 (Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14))
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别名
- CD258 / LIGHT
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背景
- LIGHT is a type II membrane protein belonging to the TNF family of membrane-anchored ligands. It is expressed in splenocytes, activated PBL, CD8+ tumor infiltrating lymphocytes, granulocytes and monocytes. LIGHT has the ability to activate NFkB, stimulate proliferation of lymphocytes, and induce apoptosis in certain human tumor cells. LIGHT binds to three receptors, the herpes virus entry mediator type A receptor (HEVM), LTbR and a decoy receptor (DcR3).Synonyms: HVEM-L, HVEML, Herpesvirus entry mediator-ligand, TNFSF14, Tumor necrosis factor ligand superfamily member 14
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基因ID
- 8740
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NCBI登录号
- NP_003798
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UniProt
- O43557
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途径
- Cancer Immune Checkpoints
抗原
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