IL-10 抗体
Quick Overview for IL-10 抗体 (ABIN116339)
抗原
See all IL-10 (IL10) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Recognizes Rat IL10. Other species not tested.
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纯化方法
- Affinity Chromatography employing immobilized Rat IL-10 matrix.
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免疫原
- Highly pure (>98%) recombinant Rat IL-10.
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anti-Interleukin 10 (IL10) (AA 101-178) antibody
IL10 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 10 (IL10) (AA 19-178) antibodyIL10 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 10 (IL10) (AA 19-178) antibodyIL10 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 10 (IL10) antibodyIL10 适用: 人, 小鼠, 大鼠, Cow, 兔, Pig, 马, 犬, 猴, 小鸡, 山羊, 绵羊, 豚鼠, Avian, Cat, 驴, Hamster WB 宿主: 山羊 Polyclonal unconjugated
anti-Interleukin 10 (IL10) antibodyMicroarray verified IL10 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB971550 unconjugated
anti-Interleukin 10 (IL10) antibodyMicroarray verified IL10 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB312926 unconjugated
anti-Interleukin 10 (IL10) antibodyIL10 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
anti-Interleukin 10 (IL10) (AA 27-53) antibodyIL10 适用: 人 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 10 (IL10) (AA 1-178) antibodyIL10 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 10 (IL10) (AA 1-178) antibodyIL10 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C5 unconjugated
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应用备注
- Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of RatIL-10 (30.00 ng/mL), a concentration of 0.45-0.80 μg/mL of this antibody is required. ELISAIndirect: To detect Rat IL-10 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant Rat IL-10. Sandwich: To detect Rat IL-10 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Rat IL-10 (PP1115B1 or PP1115B2) as a
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限制
- 仅限研究用
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溶解方式
- Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2 without preservatives.
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing. Centrifuge vial prior to opening.
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储存条件
- 4 °C/-20 °C
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储存方法
- The lyophilized antibody is stable at -20 °C for one year from despatch. The reconstituted antibody is stable for two weeks at 2-8 °C. Frozen aliquots are stable for six months when stored at -20 °C.
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有效期
- 12 months
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- IL-10 (IL10) (Interleukin 10 (IL10))
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别名
- Interleukin-10 / IL10
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背景
- Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. ILs have a variety of functions, but most function to direct other immune cells to divide and differentiate. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Human IL10 is a non glycosylated polypeptide consisting of 178 amino acids. There is 73 % homology between the human and mouse IL10 proteins, however, the human IL10 acts on both human and mouse target cells, while the mouse IL10 has species specific activity. The cellular sources of IL10 are CD4+ T cells and T cell clones, thymocytes, B cells and B cell lymphomas, macrophages, mast cell lines and keratinocytes. IL10 will stimulate the growth of stem cells, mast cells and thymocytes. IL10 enhances cytotoxic T cell development, and costimulates B cell differentiation and immunoglobulin secretion. IL10 inhibits cytokine production by macrophages and suppresses macrophage class II MHC expression. The human IL10 gene is on human chromosome 1.Synonyms: CSIF, Cytokine synthesis inhibitory factor, IL-10, TGIF
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基因ID
- 25325
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NCBI登录号
- NP_036986
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UniProt
- P29456
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途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints
抗原
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