CXCL12 抗体 (Biotin)
Quick Overview for CXCL12 抗体 (Biotin) (ABIN116231)
抗原
See all CXCL12 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody reacts with Stromal Cell Derived Factor-1 beta (SDF-1 beta).
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纯化方法
- Affinity Chromatography.
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免疫原
- Highly pure (>98%) E.coli derived recombinant Human SDF1-beta.
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亚型
- IgG
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应用备注
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ELISA: To detect hSDF-1-beta by direct ELISA (using 100 l/well antibody solution) thisantibody can be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction withcompatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinanthSDF-1-beta. Western Blot: To detect hSDF-1-beta by Western Blot analysis this antibody can be used ata concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hSDF-1-beta is 1.5 - 3.0 ng/lane, under eitherreducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Centrifuge vial prior to opening! Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2 without preservatives.
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))
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别名
- CXCL12 / SDF1
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背景
- SDF1 alpha and 1 beta are small cytokines that belong to the intercrine family, members of which activate leukocytes and are often induced by proinflammatory stimuli such as lipopolysaccharide, TNF or IL1. SDF1 alpha has been shown to be chemotactic for lymphocytes and is recently reported to be a ligand for CXCR4 (LESTR/fusin), a co-receptor for HIV1 entry into T cells. SDF-1 binding to CXCR4 inhibits HIV1 entry.Synonyms: C-X-C motif chemokine 12, IRH, Intercrine reduced in hepatomas, PBSF, Pre-B cell growth-stimulating factor, SDF1A, SDF1B, Stromal cell-derived factor 1, hIRH
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基因ID
- 6387
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NCBI登录号
- NP_000600
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UniProt
- P48061
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途径
- Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling
抗原
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