KIT Ligand 抗体
-
- 抗原 See all KIT Ligand (KITLG) 抗体
- KIT Ligand (KITLG)
-
适用
- 人
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This KIT Ligand antibody is un-conjugated
-
应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func)
- 特异性
- Human Stem Cell Factor (SCF).
- 纯化方法
- Affinity chromatography.
- 免疫原
- Highly pure (>98%) recombinant hSCF (anti-hSCF).
- Top Product
- Discover our top product KITLG Primary Antibody
-
-
- 应用备注
-
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhSCF (10 ng/mL), a concentration of 8 - 12 ng/mL of this antibody is required. ELISA: To detect hSCF by direct ELISA (using 100 l/well antibody solution) a concentrationof at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, inconjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/wellof recombinant hSCF. Western Blot: To detect hSCF by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hSCF is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions. Immunohistochemistry: 1 μg/mL is the recommended starting dilution for frozen andparaffin sections. For paraffin sections antigen retrieval techniques may be necesserary toimprove staining.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
-
- 溶解方式
- Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
- 缓冲液
- PBS, pH 7.2 without preservatives.
- 储存液
- Without preservative
- 注意事项
- Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
-
- 抗原
- KIT Ligand (KITLG)
- 别名
- KITLG / SCF (KITLG 产品)
- 别名
- Xkl-1 antibody, Xsl antibody, Xsl-1 antibody, Xsl-2 antibody, steel antibody, KITLG antibody, SCF antibody, kitl antibody, kl-1 antibody, mgf antibody, scf antibody, FPH2 antibody, KL-1 antibody, Kitl antibody, MGF antibody, SF antibody, SHEP7 antibody, Clo antibody, Con antibody, Gb antibody, Kitlg antibody, Mgf antibody, SLF antibody, Sl antibody, blz antibody, contrasted antibody, 710-712 antibody, CSF antibody, KITL antibody, KIT ligand L homeolog antibody, KIT ligand antibody, kit ligand antibody, kitlg.L antibody, KITLG antibody, kitlg antibody, Kitl antibody, Kitlg antibody
- 背景
- Synonyms: KITL, Kit ligand, MGF, Mast cell growth factor, Stem cell factor, c-Kit ligand
- 基因ID
- 4254
- NCBI登录号
- NP_000890
- UniProt
- P21583
- 途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
-