PDGFA 抗体
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北京 101111
Quick Overview for PDGFA 抗体 (ABIN116208)
抗原
See all PDGFA 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Human Platelet Derived Growth Factor AA (hPDGF-AA).
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纯化方法
- Affinity Chromatography using an immobilized Human PDGF-AA matrix.
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免疫原
- Highly pure (>98%) E.coli derived recombinant Human PDGF-AA (Cat.-No PA118).
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anti-Platelet Derived Growth Factor A (PDGFA) (AA 125-170) antibody
PDGFA 适用: 人, 小鼠 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Platelet Derived Growth Factor A (PDGFA) (AA 1-211) antibodyPDGFA 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Platelet Derived Growth Factor A (PDGFA) (AA 1-211) antibodyPDGFA 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Platelet Derived Growth Factor A (PDGFA) antibodyPDGFA 适用: 人, 小鼠, 大鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Platelet Derived Growth Factor A (PDGFA) (AA 60-89), (N-Term) antibodyPDGFA 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB01999 unconjugated
anti-Platelet Derived Growth Factor A (PDGFA) antibodyPDGFA 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Platelet Derived Growth Factor A (PDGFA) (AA 84-193) antibodyPDGFA 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Platelet Derived Growth Factor A (PDGFA) (AA 1-211) antibodyPDGFA 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Platelet Derived Growth Factor A (PDGFA) (AA 87-211) antibodyPDGFA 适用: 人, 小鼠, 大鼠 WB, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Neutralisation: To yield one-half maximal inhibition [ND50] of the biological activity ofhPDGF-AA (3.0 ng/mL), a concentration of 0.03 - 0.05 μg/mL of this antibody is required. Immunohistochemistry: A starting dilution of 1 μg/mL is recommended for frozen sections. ELISA: To detect hPDGF-AA by direct ELISA (using 100l/well antibody solution) aconcentration of at least 0.5 g/ ml of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2 - 0.4 ng/well of recombinant hPDGF-AA. Western Blot: To detect hPDGF-AA by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.5 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hPDGF-AA is 1.5 -3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2 without preservatives.
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- PDGFA (Platelet Derived Growth Factor A (PDGFA))
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别名
- PDGFA
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背景
- PDGF is a mitogen for mesenchyme- and glia-derived cells. It consists of two disulfide-bonded polypeptide chains, A and B, and occurs as three isoforms, PDGF AA, PDGF AB and PDGF BB. The three isoforms bind with different affinities to two receptor types, A and B, which are structurally related and endowed with protein-tyrosine kinase domains. Ligand binding induces activation of the receptor kinases by formation of receptor dimers, the A subunit of PDGF binds only to A receptors with high affinity, whereas the B subunit can bind to both A and B receptors. Evidence suggests that PDGF may function as a neurotrophic factor. The fact that PDGF-A receptors are expressed in oligodendrocyte progenitor cells, whereas PDGF-B receptors are expressed on neurons, suggests that the different isoforms of PDGF may regulate growth and differentiation of different cell types in the developing central nervous system by paracrine and autocrine routes.Synonyms: PDGF A, PDGF alpha, PDGF subunit A, PDGF-1, PDGF-A, PDGF1, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha, Platelet-derived growth factor subunit A
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基因ID
- 5154
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NCBI登录号
- NP_002598
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UniProt
- P04085
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途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling
抗原
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