PDGFA 抗体
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- 抗原 See all PDGFA 抗体
- PDGFA (Platelet Derived Growth Factor A (PDGFA))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This PDGFA antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)
- 特异性
- The antibody recognizes the human Platelet Derived Growth Factor AA (hPDGF-AA). Not tested in other species.
- 纯化方法
- Immunoaffinity chromatography using immobilized human PDGF matrix
- 免疫原
- Highly pure (>98%) recombinant hPDGF-AA
- Top Product
- Discover our top product PDGFA Primary Antibody
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anti-Platelet Derived Growth Factor A (PDGFA) (AA 125-170) antibody
PDGFA 适用: 人, 小鼠 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Platelet Derived Growth Factor A (PDGFA) (AA 1-211) antibodyPDGFA 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Platelet Derived Growth Factor A (PDGFA) antibodyPDGFA 适用: 人, 小鼠, 大鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Platelet Derived Growth Factor A (PDGFA) (AA 1-211) antibodyPDGFA 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Platelet Derived Growth Factor A (PDGFA) (Internal Region) antibodyPDGFA 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Platelet Derived Growth Factor A (PDGFA) antibodyPDGFA 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Platelet Derived Growth Factor A (PDGFA) (AA 84-193) antibodyPDGFA 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Platelet Derived Growth Factor A (PDGFA) (AA 1-211) antibodyPDGFA 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Platelet Derived Growth Factor A (PDGFA) (AA 60-89), (N-Term) antibodyPDGFA 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB01999 unconjugated
anti-Platelet Derived Growth Factor A (PDGFA) (Center) antibodyPDGFA 适用: 人, 小鼠, 大鼠, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Neutralisation: To yield one-half maximal inhibition [ND50] of the biological activity of hPDGF-AA (5.0 ng/mL),a concentration of 0.05 - 0.07 μg/mL of this antibody is required. Immunohistochemistry: The antibody stained formalin-fixed, paraffin embedded sections of normal human skin at1.0 μg/mL overnight at 4C. (A HRP-labeled polymer detection system was used with anon-alcohol soluble AEC chromogen and a proteinase K antigen retrieval). ELISA: Indirect - To detect hPDGF-AA by indirect ELISA (using 100 μL/well antibody solution),a concentration of 0.5 - 2.0 μg/mL of this antibody is required, allows the detection of atleast 0.2 - 0.4 ng/well of recombinant hPDGF-AA. Sandwich - To detect hPDGF-AA by sandwich ELISA (using 100 μL/well antibody solution),a concentration of 0.5 - 2.0 μg/mL of this antibody is required, in conjunction with abiotinylated anti-human PDGF-AA (PP1061B) as the detection antibody at least 0.2 - 0.4 ng/well of recombinant hPDGF-AA can be detected. Western blot: To detect hPDGF-AA by Western blot analysis this antibody can be used at a concentrationof 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hPDGF-AA is 1.5 -3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
- 缓冲液
- PBS, pH 7.2, without preservatives
- 储存液
- Without preservative
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The lyophilized antibody is stable at -20 °C for one year from despatch. The reconstituted antibody can be stored for two weeks at 2-8 °C. Frozen aliquots are stable for six months when stored at -20 °C.
- 有效期
- 12 months
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- 抗原
- PDGFA (Platelet Derived Growth Factor A (PDGFA))
- 别名
- PDGFA (PDGFA 产品)
- 别名
- PDGF-A antibody, PDGF1 antibody, pdgfa antibody, pdgfa-2 antibody, zgc:100777 antibody, pdgf antibody, pdgf1 antibody, pdgf-a antibody, AA409063 antibody, CD142 antibody, Cf-3 antibody, Cf3 antibody, TF antibody, PDGFACP antibody, wu:fc32c12 antibody, zgc:153249 antibody, platelet derived growth factor subunit A antibody, platelet-derived growth factor alpha polypeptide a antibody, coagulation factor III antibody, platelet derived growth factor, alpha antibody, platelet derived growth factor subunit A S homeolog antibody, platelet-derived growth factor alpha polypeptide b antibody, PDGFA antibody, pdgfaa antibody, pdgfa antibody, F3 antibody, Pdgfa antibody, pdgfa.S antibody, pdgfab antibody
- 背景
- Synonyms: PDGF A, PDGF alpha, PDGF subunit A, PDGF-1, PDGF-A, PDGF1, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha, Platelet-derived growth factor subunit A
- 基因ID
- 5154
- NCBI登录号
- NP_002598
- UniProt
- P04085
- 途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling
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