PDGFA 抗体 (Biotin)
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- 抗原 See all PDGFA 抗体
- PDGFA (Platelet Derived Growth Factor A (PDGFA))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This PDGFA antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- 特异性
- Human Platelet Derived Growth Factor AA (hPDGF-AA).
- 纯化方法
- Affinity Chromatography
- 免疫原
- Highly pure (>98%) E.coli derived recombinant Human PDGF-AA (Cat.-No PA118)
- Top Product
- Discover our top product PDGFA Primary Antibody
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- 应用备注
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ELISA: To detect hPDGF-AA by direct ELISA (using 100 l/well antibody solution) thisantibody can be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction withcompatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinanthPDGF-AA. Western Blot: To detect hPDGF-AA by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hPDGF-AA is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
- 缓冲液
- PBS, pH 7.2 without preservatives
- 储存液
- Without preservative
- 注意事项
- Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.
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- 抗原
- PDGFA (Platelet Derived Growth Factor A (PDGFA))
- 别名
- PDGFA (PDGFA 产品)
- 别名
- PDGF-A antibody, PDGF1 antibody, pdgfa antibody, pdgfa-2 antibody, zgc:100777 antibody, pdgf antibody, pdgf1 antibody, pdgf-a antibody, AA409063 antibody, CD142 antibody, Cf-3 antibody, Cf3 antibody, TF antibody, PDGFACP antibody, wu:fc32c12 antibody, zgc:153249 antibody, platelet derived growth factor subunit A antibody, platelet-derived growth factor alpha polypeptide a antibody, coagulation factor III antibody, platelet derived growth factor, alpha antibody, platelet derived growth factor subunit A S homeolog antibody, platelet-derived growth factor alpha polypeptide b antibody, PDGFA antibody, pdgfaa antibody, pdgfa antibody, F3 antibody, Pdgfa antibody, pdgfa.S antibody, pdgfab antibody
- 背景
- PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs, PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet alpha-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-alpha and PDGFR-beta. PDGFRalpha is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB.Synonyms: PDGF A, PDGF alpha, PDGF subunit A, PDGF-1, PDGF-A, PDGF1, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha, Platelet-derived growth factor subunit A
- 基因ID
- 5154
- NCBI登录号
- NP_002598
- UniProt
- P04085
- 途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling
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