CCL19 抗体 (Biotin)
Quick Overview for CCL19 抗体 (Biotin) (ABIN116173)
抗原
See all CCL19 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Human Macrophage Inflammatory Protein-3 beta (MIP-3 beta).
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纯化方法
- Affinity chromatography.
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免疫原
- Highly pure (>98%) recombinant hMIP-3 beta (human MIP-3 beta).
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应用备注
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ELISA: To detect hMIP-3 beta by direct ELISA (using 100 l/well antibody solution) thisantibody can be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction withcompatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinanthMIP-3 betaWestern Blot: To detect hMIP-3 beta by Western Blot analysis this antibody can be used ata concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hMIP-3 beta is 1.5 - 3.0 ng/lane, under eitherreducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2 without preservatives.
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
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储存条件
- 4 °C/-20 °C
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储存方法
- Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.
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- CCL19 (Chemokine (C-C Motif) Ligand 19 (CCL19))
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别名
- MIP3-beta / CCL19
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背景
- MIP3 chemokines are among many novel beta chemokines recently identified through bioinformatics in the Expressed Sequence Tag (EST) database. They are distantly related to other beta chemokines (20-30 % aa sequence homology). MIP3 chemokines are useful probes for studying the biology of inflammation and cell migration. Their expression is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL10. MIP3 beta is also known as ELC (EBI1-1 ligand chemokine) and is constitutively expressed in various lymphoid tissues (thymus, lymph nodes, appendix and spleen). MIP3 beta is a chemoattractant for cultured human lymphocytes, dendritic cells, human T lymphoblastoid CEM NKR2 and hemopoietic progenitor cells. It may be involved in G Protein activation in human NK cells. MIP3 beta is a unique functional ligand for CCR7. The MIP3 beta gene has been mapped to chormosome 9p13.Synonyms: Beta chemokine exodus-3, C-C motif chemokine 19, CCL-19, CK beta-11, EBI1-ligand chemokine, ELC, Exodus-3, MIP-3 beta, MIP3B, Macrophage inflammatory protein 3 beta, SCYA19, Small-inducible cytokine A19, Small-inducible cytokine A19
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基因ID
- 6363
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NCBI登录号
- NP_006265
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UniProt
- Q99731
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途径
- Positive Regulation of Immune Effector Process
抗原
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