M-CSF/CSF1 抗体
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北京 101111
Quick Overview for M-CSF/CSF1 抗体 (ABIN116156)
抗原
See all M-CSF/CSF1 (CSF1) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Human Macrophage Colony Stimulating Factor (M-CSF)
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纯化方法
- Affinity chromatography.
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免疫原
- Highly pure (>98%) recombinant hM-CSF (human M-CSF).
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应用备注
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Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhM-CSF (7.0 ng/mL), a concentration of 0.02-0.03 μg/mL of this antibody is required. ELISA: (Indirect): To detect hM-CSF by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hM-CSF. (Sandwich): To detect hM-CSF by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Human M-CSF (PP1048B) as a detectionantibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hM-CSF. Western Blot: To detect hM-CSF by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hM-CSF is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2 without preservatives.
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储存液
- Without preservative
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注意事项
- Centrifuge vial prior to opening!
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
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- M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))
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别名
- Macrophage Colony Stimulating Factor (M-CSF)
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背景
- Synonyms: CSF-1, CSF1, MCSF, Macrophage colony-stimulating factor 1
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基因ID
- 1435
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NCBI登录号
- NP_000748
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UniProt
- P09603
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途径
- RTK signaling
抗原
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