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M-CSF/CSF1 抗体

The 兔 多克隆 anti-M-CSF/CSF1 antibody is suitable to detect M-CSF/CSF1 in samples from 人. It has been validated for WB, IHC (p), EIA 和 Func.
产品编号 ABIN116156
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for M-CSF/CSF1 抗体 (ABIN116156)

抗原

See all M-CSF/CSF1 (CSF1) 抗体
M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

适用

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宿主

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克隆类型

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多克隆

标记

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This M-CSF/CSF1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)
  • 特异性

    Human Macrophage Colony Stimulating Factor (M-CSF)

    纯化方法

    Affinity chromatography.

    免疫原

    Highly pure (>98%) recombinant hM-CSF (human M-CSF).
  • 应用备注

    Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhM-CSF (7.0 ng/mL), a concentration of 0.02-0.03 μg/mL of this antibody is required. ELISA: (Indirect): To detect hM-CSF by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hM-CSF. (Sandwich): To detect hM-CSF by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Human M-CSF (PP1048B) as a detectionantibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hM-CSF. Western Blot: To detect hM-CSF by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hM-CSF is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 溶解方式

    Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

    缓冲液

    PBS, pH 7.2 without preservatives.

    储存液

    Without preservative

    注意事项

    Centrifuge vial prior to opening!

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
  • 抗原

    M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

    别名

    Macrophage Colony Stimulating Factor (M-CSF)

    背景

    Synonyms: CSF-1, CSF1, MCSF, Macrophage colony-stimulating factor 1

    基因ID

    1435

    NCBI登录号

    NP_000748

    UniProt

    P09603

    途径

    RTK signaling
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