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CCL2 抗体

CCL2 适用: 人 WB, IHC (p), EIA, Func 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN116140
发货至: 中国
  • 抗原 See all CCL2 抗体
    CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))
    适用
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    克隆类型
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    多克隆
    标记
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    This CCL2 antibody is un-conjugated
    应用范围
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    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)
    纯化方法
    Affinity Chromatography.
    免疫原
    Highly pure (>98%) E.coli derived recombinant Human MCP-1 (MCAF).
    Top Product
    Discover our top product CCL2 Primary Antibody
  • 应用备注
    Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhMCP-1(MCAF) (10.0 ng/mL), a concentration of 3.0-5.0 μg/mL of this antibody is required. Sandwich ELISA: To detect Human MCP-1(MCAF) by Sandwich ELISA (using 100 μL/wellantibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. Thisantigen affinity purified antibody, in conjunction with Biotinylated Anti-Human MCP-1(MCAF) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well ofrecombinant hMCP-1(MCAF). Western Blot: To detect Human MCP-1 (MCAF) by Western Blot analysis this antibody canbe used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatiblesecondary reagents the detection limit for recombinant Human MCP-1(MCAF) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Immunohistochemistry (2.5 μg/mL for 2hrs at RT). This antibody stained Formalin-Fixed, Paraffin-Embedded Sections of Human breastinvasive ductal carcinoma. An HRP-labeled polymer detection system was used with a DABChromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer isrecommended.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 溶解方式
    Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
    缓冲液
    PBS, pH 7.2 without preservatives.
    储存液
    Without preservative
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Store the antibody in aliquots at -20 °C.
  • 抗原
    CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))
    别名
    MCP1 / CCL2 (CCL2 产品)
    别名
    GDCF-2 antibody, HC11 antibody, HSMCR30 antibody, MCAF antibody, MCP-1 antibody, MCP1 antibody, SCYA2 antibody, SMC-CF antibody, AI323594 antibody, JE antibody, Scya2 antibody, Sigje antibody, MCP-1A antibody, MCP1A antibody, C-C motif chemokine ligand 2 antibody, chemokine (C-C motif) ligand 2 antibody, C-C motif chemokine 2 antibody, CCL2 antibody, Ccl2 antibody, LOC101120093 antibody
    背景
    This chemotactic factor attracts monocytes and basophils but not neutrophils or eosinophils and augments monocyte anti-tumor activity. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. It may be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.Synonyms: C-C motif chemokine 2, HC-11, HC11, MCAF, MCP-1, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, SCYA2, Small-inducible cytokine A2
    基因ID
    9606
    UniProt
    P13500
    途径
    Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
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