CCL2 抗体
Quick Overview for CCL2 抗体 (ABIN116139)
抗原
See all CCL2 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Affinity Chromatography.
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免疫原
- Highly pure (>98%) E.coli derived recombinant Human MCP-1 (MCAF).
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anti-Chemokine (C-C Motif) Ligand 2 (CCL2) antibody
CCL2 适用: 人, 小鼠, 猴 WB, IHC, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 2D8 unconjugated
anti-Chemokine (C-C Motif) Ligand 2 (CCL2) (AA 61-148) antibodyCCL2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (p), IHC (fro), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 2 (CCL2) antibodyCCL2 适用: 人 WB, IHC, ELISA, IHC (p), IF, FACS 宿主: 小鼠 Monoclonal 2D8 unconjugated
anti-Chemokine (C-C Motif) Ligand 2 (CCL2) (AA 24-96) antibodyCCL2 适用: 小鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C1 unconjugated
anti-Chemokine (C-C Motif) Ligand 2 (CCL2) (AA 62-89), (C-Term) antibodyCCL2 适用: 人 WB, IHC (p), IF, FACS 宿主: 兔 Polyclonal RB17838 unconjugated
anti-Chemokine (C-C Motif) Ligand 2 (CCL2) (AA 62-89) antibodyCCL2 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 2 (CCL2) antibodyCCL2 适用: 人, 小鼠, 猴 WB, IHC (p), IF, FACS, EIA 宿主: 小鼠 Monoclonal 2D8 unconjugated
anti-Chemokine (C-C Motif) Ligand 2 (CCL2) (AA 31-80) antibodyCCL2 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 2 (CCL2) (AA 24-99) antibodyCCL2 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 2 (CCL2) antibodyCCL2 适用: 小鼠 WB, ELISA, Neut 宿主: 兔 Polyclonal unconjugated
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应用备注
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Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhMCP-1(MCAF) (10.0 ng/mL), a concentration of 3.0-5.0 μg/mL of this antibody is required. Sandwich ELISA: To detect Human MCP-1(MCAF) by Sandwich ELISA (using 100 μL/wellantibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. Thisantigen affinity purified antibody, in conjunction with Biotinylated Anti-Human MCP-1(MCAF) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well ofrecombinant hMCP-1(MCAF). Western Blot: To detect Human MCP-1 (MCAF) by Western Blot analysis this antibody canbe used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatiblesecondary reagents the detection limit for recombinant Human MCP-1(MCAF) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Immunohistochemistry (2.5 μg/mL for 2hrs at RT). This antibody stained Formalin-Fixed, Paraffin-Embedded Sections of Human breastinvasive ductal carcinoma. An HRP-labeled polymer detection system was used with a DABChromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer isrecommended.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2 without preservatives.
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the antibody in aliquots at -20 °C or below.
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- CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))
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别名
- MCP1 / CCL2
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背景
- This chemotactic factor attracts monocytes and basophils but not neutrophils or eosinophils and augments monocyte anti-tumor activity. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. It may be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.Synonyms: C-C motif chemokine 2, HC-11, HC11, MCAF, MCP-1, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, SCYA2, Small-inducible cytokine A2
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基因ID
- 9606
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UniProt
- P13500
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途径
- Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
抗原
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