Leptin 抗体
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北京 101111
Quick Overview for Leptin 抗体 (ABIN116132)
抗原
See all Leptin (LEP) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody reacts with Human Leptin.
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纯化方法
- Affinity chromatography.
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免疫原
- Highly pure (>98%) recombinant hLeptin
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anti-Leptin (LEP) (AA 22-167) antibody
LEP 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Leptin (LEP) (AA 22-167) antibodyLEP 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Leptin (LEP) (AA 17-100) antibodyLEP 适用: 人, 小鼠, 大鼠 WB, ELISA, IF (p), IF (cc), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Leptin (LEP) (AA 8-37) antibodyLEP 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Leptin (LEP) (AA 22-167) antibodyLEP 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Leptin (LEP) (AA 29-160) antibodyLEP 适用: Cow WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Leptin (LEP) (AA 7-37), (N-Term) antibodyLEP 适用: 人 WB, IF, FACS, EIA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Leptin (LEP) (AA 101-154) antibodyLEP 适用: 小鼠, 大鼠 ELISA, IF (p), IF (cc), FACS, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Leptin (LEP) (AA 22-167) antibodyLEP 适用: 犬 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Leptin (LEP) (AA 22-167) antibodyLEP 适用: 人 WB 宿主: 小鼠 Monoclonal C5 unconjugated
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应用备注
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Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhLeptin (7.5 ng/mL), a concentration of 0.5 - 3.0 μg/mL of this antibody is required. ELISA: To detect hLeptin by direct ELISA (using 100l/well antibody solution) aconcentration of at least 0.5g/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 -0.4 ng/well of recombinant hLeptin. Western Blot: To detect hLeptin by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hLeptin is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions. Immunohistochemistry: use at approx. 2 μg/mL for cryostat and paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2 without preservatives.
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- Leptin (LEP)
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别名
- Leptin
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背景
- Leptin plays a critical role in the regulation of body weight by inhibiting food intake and stimulating energy expenditure. Defects in Leptin production cause severe hereditary obesity in rodents and humans. In addition to its effects on body weight, leptin has a variety of other functions, including the regulation of hematopoiesis, angiogenesis, wound healing, and the immune and inflammatory response. The Leptin gene is the human homolog of the gene (ob) mutant in the mouse 'obese' phenotype. Defects in the Leptin gene are the cause of profound obesity and type II diabetes.Synonyms: LEP, OB, OBS, Obese protein, Obesity factor
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基因ID
- 3952
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NCBI登录号
- NP_000221
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UniProt
- P41159
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途径
- JAK/STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process
抗原
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