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FGF7 抗体

This anti-FGF7 antibody is a 兔 多克隆 antibody detecting FGF7 in WB, IHC (p), EIA 和 Func. Suitable for 人. This Primary Antibody has been cited in 1 publication.
产品编号 ABIN116127
发货至: 中国

Quick Overview for FGF7 抗体 (ABIN116127)

抗原

See all FGF7 抗体
FGF7 (Fibroblast Growth Factor 7 (FGF7))

适用

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宿主

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克隆类型

  • 67
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多克隆

标记

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This FGF7 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)
  • 特异性

    This antibody reacts with Human Keratinocyte Growth Factor (KGF).

    纯化方法

    Affinity chromatography

    免疫原

    Highly purified (>98%) recombinant FGF-7 (KGF)
  • 应用备注

    Neutralisation: To yield one-half maximal inhibition [ND50]of the biological activity of hKGF(25 ng/mL), a concentration of 0.56-0.85 μg/mL of this antibody has been used for previouslots. ELISA: To detect hKGF by direct ELISA (using 100 l/well antibody solution) a concentrationof at least 0.5g/mL of this antibody is required. This antigen affinity purified antibody, inconjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/wellof recombinant hKGF. Western blot: To detect hKGF by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hKGF is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 溶解方式

    Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

    缓冲液

    PBS, pH 7.2 without preservatives

    储存液

    Without preservative

    注意事项

    Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
  • Lim, Poh, Riau, Beuerman, Tan, Mehta: "Biological and ultrastructural properties of acelagraft, a freeze-dried γ-irradiated human amniotic membrane." in: Archives of ophthalmology, Vol. 128, Issue 10, pp. 1303-10, (2010) (PubMed).

  • 抗原

    FGF7 (Fibroblast Growth Factor 7 (FGF7))

    别名

    FGF7 / KGF

    背景

    KGF is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis.Synonyms: Fibroblast growth factor 7, HBGF7, Heparin-binding growth factor 7, Keratinocyte growth factor

    基因ID

    2252

    NCBI登录号

    NP_002000

    UniProt

    P21781

    途径

    RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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