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IL-7 抗体 (Biotin)

This Biotin-conjugated 兔 多克隆 anti-IL-7 antibody specifically detects IL-7 in WB 和 EIA. The antibody is reactive with 人 samples.
产品编号 ABIN116086
发货至: 中国
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北京 101111
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Room 801-803
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Quick Overview for IL-7 抗体 (Biotin) (ABIN116086)

抗原

See all IL-7 (IL7) 抗体
IL-7 (IL7) (Interleukin 7 (IL7))

适用

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宿主

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克隆类型

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多克隆

标记

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This IL-7 antibody is conjugated to Biotin

应用范围

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Western Blotting (WB), Enzyme Immunoassay (EIA)
  • 特异性

    Human IL-7

    纯化方法

    Affinity chromatography.

    免疫原

    Highly pure (>98%) recombinant hIL-7 (human IL-7).
  • 应用备注

    ELISA: To detect hIL-7 by direct ELISA (using 100 ml/well antibody solution) this antibodycan be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant hIL-7. Western Blot: To detect hIL-7 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 mg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hIL-7 is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 溶解方式

    Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

    缓冲液

    PBS, pH 7.2 without preservatives.

    储存液

    Without preservative

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody at 4-8 °C for one month or at -20 °C for longer.
  • 抗原

    IL-7 (IL7) (Interleukin 7 (IL7))

    别名

    Interleukin-7 / IL7

    背景

    Interleukin 7 (IL7) is a lymphoid cell growth factor that affects pre-B, pro-B, and early T cells. IL7 was previously known as pre-B cell growth factor and lymphopoietin 1. IL7 supports the growth of early B cells from long-term lymphoid bone marrow cultures. It is mitogenic to thymocytes and enhances the response of cells to other stimuli such as polyhydroxyalkanoate (PHA) and concanavalin A (ConA). IL7 stimulates the proliferation of CD4+/CD8+ cells. The proliferative response of thymocytes to IL7 is not affected by antibodies to the T cell growth factors such as IL2, IL4 and IL6, suggesting that IL7 is capable of stimulating T cell proliferation through a pathway independent of the known T cell growth factors. Mature T cells respond to IL7 and Con A, but not to IL7 alone. In myeloid lineage cells, IL7 upregulates the production of pro-inflammatory cytokines and stimulates the tumoricidal activity of monocytes/macrophages. IL7 is expressed by adherent stromal cells from various tissues.Synonyms: IL-7

    基因ID

    3574

    NCBI登录号

    NP_000871

    UniProt

    P13232

    途径

    JAK/STAT Signaling
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