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IL-3 抗体

IL-3 适用: 人 WB, EIA, Func 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN116071
发货至: 中国
  • 抗原 See all IL-3 抗体
    IL-3 (Interleukin 3 (IL-3))
    适用
    • 72
    • 36
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    宿主
    • 75
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    克隆类型
    • 81
    • 36
    多克隆
    标记
    • 65
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    This IL-3 antibody is un-conjugated
    应用范围
    • 87
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    Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)
    特异性
    Specific for human IL-3.
    纯化方法
    Affinity chromatography.
    免疫原
    Highly pure (>98%) recombinant hIL-3.
    Top Product
    Discover our top product IL-3 Primary Antibody
  • 应用备注
    Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-3(3.0 ng/mL), a concentration of 0.06 - 0.1 μg/mL of this antibody is required. ELISA: To detect hIL-3 by direct ELISA (using 100 l/well antibody solution) a concentrationof at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, inconjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/wellof recombinant hIL-3. Western Blot: To detect hIL-3 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hIL-3 is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 溶解方式
    Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
    缓冲液
    PBS, pH 7.2 without preservatives.
    储存液
    Without preservative
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C/-20 °C
    储存方法
    Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
  • 抗原
    IL-3 (Interleukin 3 (IL-3))
    别名
    Interleukin-3 / IL3 (IL-3 产品)
    别名
    BPA antibody, Csfmu antibody, HCGF antibody, Il-3 antibody, MCGF antibody, PSF antibody, IL-3 antibody, MULTI-CSF antibody, interleukin 3 antibody, interleukin 3 (colony-stimulating factor, multiple) antibody, Il3 antibody, IL3 antibody
    背景
    The pleiotropic IL3 (Interleukin 3) is a 15 kDa cytokine that is primarily secreted by activated T lymphocytes and stimulates the proliferation and differentiation of hematopoietic cells. IL3 acts in hematopoiesis by controlling the production, differentiation, and function of two related white cell populations of the blood, the granulocytes and the monocytes/macrophages. It induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes and is expressed by activated T cells, mast cells, and natural killer cells. IL3 not only supports growth of both pluripotent stem cells and the more differentiated committed progenitors, but it also stimulates the functional activity of some fully differentiated cells. IL3 has also been shown to protect mast cells from undergoing apoptosis.Synonyms: Hematopoietic growth factor, IL-3, MCGF, Mast cell growth factor, Multipotential colony-stimulating factor, P-cell-stimulating factor
    基因ID
    3562
    NCBI登录号
    NP_000579
    UniProt
    P08700
    途径
    JAK/STAT Signaling, Regulation of Carbohydrate Metabolic Process, Autophagy
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