IL-3 抗体 (Biotin)
Quick Overview for IL-3 抗体 (Biotin) (ABIN116069)
抗原
See all IL-3 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Specific for Human IL-3
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纯化方法
- Affinity chromatography.
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免疫原
- Highly pure (>98%) recombinant human IL-3.
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anti-Interleukin 3 (IL-3) antibody
IL-3 适用: 小鼠 WB, ELISA, Neut 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 3 (IL-3) (AA 101-166) antibodyIL-3 适用: 小鼠, 大鼠 WB, ELISA, IF (p), IF (cc), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 3 (IL-3) (AA 20-152) antibodyIL-3 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 3 (IL-3) antibodyIL-3 适用: 大鼠 WB, ELISA, Neut 宿主: 山羊 Polyclonal unconjugated
anti-Interleukin 3 (IL-3) (AA 33-166) antibodyIL-3 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 3 (IL-3) (AA 1-152) antibodyIL-3 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 3 (IL-3) (AA 1-152) antibodyIL-3 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Interleukin 3 (IL-3) antibody (Biotin)IL-3 适用: 人 WB, ELISA, Neut 宿主: 兔 Polyclonal Biotin
anti-Interleukin 3 (IL-3) antibody (Biotin)IL-3 适用: 小鼠 WB, ELISA, Neut 宿主: 兔 Polyclonal Biotin
anti-Interleukin 3 (IL-3) (AA 20-152) antibodyIL-3 适用: 人 WB, IHC, IF 宿主: 小鼠 Monoclonal 3B11 unconjugated
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应用备注
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ELISA: To detect hIL-3 by direct ELISA (using 100 l/well antibody solution) this antibodycan be used at a concentration of 0.15-0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant hIL-3. Western Blot: To detect hIL-3 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hIL-3 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2 without preservatives.
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- IL-3 (Interleukin 3 (IL-3))
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别名
- Interleukin-3 / IL3
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背景
- The pleiotropic IL3 (Interleukin 3) is a 15 kDa cytokine that is primarily secreted by activated T lymphocytes and stimulates the proliferation and differentiation of hematopoietic cells. IL3 acts in hematopoiesis by controlling the production, differentiation, and function of two related white cell populations of the blood, the granulocytes and the monocytes/macrophages. It induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes and is expressed by activated T cells, mast cells, and natural killer cells. IL3 not only supports growth of both pluripotent stem cells and the more differentiated committed progenitors, but it also stimulates the functional activity of some fully differentiated cells. IL3 has also been shown to protect mast cells from undergoing apoptosis.Synonyms: Hematopoietic growth factor, IL-3, MCGF, Mast cell growth factor, Multipotential colony-stimulating factor, P-cell-stimulating factor
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基因ID
- 3562
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NCBI登录号
- NP_000579
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UniProt
- P08700
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途径
- JAK/STAT Signaling, Regulation of Carbohydrate Metabolic Process, Autophagy
抗原
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