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Interferon gamma 抗体

IFNG 适用: 人 WB, IHC (p), Func, EIA 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN116053
发货至: 中国
  • 抗原 See all Interferon gamma (IFNG) 抗体
    Interferon gamma (IFNG)
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    标记
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    This Interferon gamma antibody is un-conjugated
    应用范围
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    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func), Enzyme Immunoassay (EIA)
    纯化方法
    Affinity Chromatography using immobilized Human IFN-gamma matrix
    免疫原
    Highly purified (>98%) E.coli derived recombinant Human Inferferon gamma (Cat.-No PA070)
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    Discover our top product IFNG Primary Antibody
  • 应用备注
    Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofh-IFN-gamma (50 pg/mL), a concentration of 0.04-0.06 μg/mL of the previous lot of thisantibody was required. ELISA: To detect h-IFN-gamma by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2-0.4 ng/well of recombinant h-IFN-gamma. Western blot: To detect h-IFN-gamma by Western Blot analysis this antibody can be used ata concentration of 0.1- 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant h-IFN-gamma is 1.5-3.0 ng/lane, under either reducingor non-reducing conditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 溶解方式
    Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
    缓冲液
    PBS, pH 7.2 without preservatives
    储存液
    Without preservative
    注意事项
    Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
    储存条件
    4 °C/-20 °C
    储存方法
    Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
  • 抗原
    Interferon gamma (IFNG)
    别名
    IFNG / Interferon gamma (IFNG 产品)
    别名
    IFG antibody, IFI antibody, IFN-g antibody, Ifg antibody, IFNG2 antibody, IFN-gamma antibody, IFN-G antibody, IFNG antibody, IFNgamma antibody, TCRalpha antibody, INF-G antibody, ifng antibody, interferon gamma antibody, interferon, gamma 1-2 antibody, IFNG antibody, Ifng antibody, ifng1-2 antibody
    背景
    Interferon gamma is mainly produced by T lymphocytes and NK cells. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses,including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak antiviral and antiproliferative activity, and poteniates the antiviral and anti tumor effects of IFN alpha / beta (type I interferon). It is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. Labile at pH 2.Synonyms: IFN-gamma, gamma IFN
    基因ID
    9606
    UniProt
    P01579
    途径
    Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy
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