Interferon gamma 抗体
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北京 101111
Quick Overview for Interferon gamma 抗体 (ABIN116053)
抗原
See all Interferon gamma (IFNG) 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Affinity Chromatography using immobilized Human IFN-gamma matrix
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免疫原
- Highly purified (>98%) E.coli derived recombinant Human Inferferon gamma (Cat.-No PA070)
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anti-Interferon gamma (IFNG) (AA 75-155) antibody
IFNG 适用: 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Interferon gamma (IFNG) (AA 81-166) antibodyIFNG 适用: 人 WB, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Interferon gamma (IFNG) (AA 23-156) antibodyIFNG 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interferon gamma (IFNG) antibody (Biotin)IFNG 适用: 人, Non-Human Primate WB, ELISA, FACS, IP, ICC, IHC (p) 宿主: 小鼠 Monoclonal 4S-B3 Biotin
anti-Interferon gamma (IFNG) antibodyIFNG 适用: 人 IHC, FACS, IF 宿主: 小鼠 Monoclonal IFNG-466 unconjugated
anti-Interferon gamma (IFNG) antibodyIFNG 适用: 人, Non-Human Primate WB, ELISA, FACS, IP, ICC, IHC (p), RIA 宿主: 小鼠 Monoclonal 4S-B3 unconjugated
anti-Interferon gamma (IFNG) antibodyMicroarray verified IFNG 适用: 人 IHC 宿主: 小鼠 Monoclonal MAB393378 unconjugated
anti-Interferon gamma (IFNG) antibodyIFNG 适用: 人 WB, IHC (p), IF (p) 宿主: 小鼠 Monoclonal F2E4 unconjugated
anti-Interferon gamma (IFNG) (AA 24-166) antibodyIFNG 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interferon gamma (IFNG) (AA 24-167) antibody (APC)IFNG 适用: 兔 WB, IHC, IP, ICC 宿主: 豚鼠 Polyclonal APC
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应用备注
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Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofh-IFN-gamma (50 pg/mL), a concentration of 0.04-0.06 μg/mL of the previous lot of thisantibody was required. ELISA: To detect h-IFN-gamma by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2-0.4 ng/well of recombinant h-IFN-gamma. Western blot: To detect h-IFN-gamma by Western Blot analysis this antibody can be used ata concentration of 0.1- 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant h-IFN-gamma is 1.5-3.0 ng/lane, under either reducingor non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2 without preservatives
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- Interferon gamma (IFNG)
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别名
- IFNG / Interferon gamma
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背景
- Interferon gamma is mainly produced by T lymphocytes and NK cells. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses,including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak antiviral and antiproliferative activity, and poteniates the antiviral and anti tumor effects of IFN alpha / beta (type I interferon). It is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. Labile at pH 2.Synonyms: IFN-gamma, gamma IFN
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基因ID
- 9606
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UniProt
- P01579
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途径
- Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy
抗原
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