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CCL1 抗体 (Biotin)

The 兔 多克隆 anti-CCL1 antibody is suitable to detect CCL1 in samples from 人. It has been validated for WB 和 EIA.
产品编号 ABIN116045
发货至: 中国
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中国
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for CCL1 抗体 (Biotin) (ABIN116045)

抗原

See all CCL1 抗体
CCL1 (Chemokine (C-C Motif) Ligand 1 (CCL1))

适用

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宿主

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克隆类型

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多克隆

标记

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This CCL1 antibody is conjugated to Biotin

应用范围

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Western Blotting (WB), Enzyme Immunoassay (EIA)
  • 特异性

    Recognizes Human I-309

    交叉反应 (详细)

    Species reactivity (tested):Human.

    纯化方法

    Affinity Chromatography.

    免疫原

    Highly pure (>98%) recombinant Human I-309. Remarks: Molecular Weight: ~150 kDa
  • 应用备注

    Direct ELISA: To detect Human I-309 by Direct ELISA (using 100 μL/well) a concentration ofapprox. 1.0 μg/mL of this antibody is required. This Biotinylated polyclonal antibody allows thedetection of at least 0.2-0.4 ng/well of recombinant Human I-309. Sandwich ELISA: To detect Human I-309 by Sandwich ELISA (using 100 μL/well) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This Biotinylated polyclonalantibody, in conjunction with anti-Human I-309 (PP1017P1 or PP1017P2) as a Captureantibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human I-309. Western Blot: To detect Human I-309 by Western Blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant Human I-309 is 1.5-3.0 ng/lane, under either reducingor non-reducing conditions.

    限制

    仅限研究用
  • 溶解方式

    Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

    缓冲液

    PBS, pH 7.2 without preservatives.

    储存液

    Without preservative

    注意事项

    Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
  • 抗原

    CCL1 (Chemokine (C-C Motif) Ligand 1 (CCL1))

    别名

    CCL1 / I-309

    背景

    I-309 is a member of the C-C, or beta chemokine class. Other chemokines in this group include MIP1 alpha, MIP1 beta, RANTES, MCP1/2/3/4, mouse JE, 1 - 2 μg/mL antibody detects 20 and mouse MARC, eotaxin, HCC1 and C10. These chemokines act primarily as chemoattractants and activate monocytes, dendritic cells, T lymphocytes, natural killer cells, B lymphocytes, basophils and eosinophils. I-309 was originally identified by subtractive hybridization as a transcript that was present in a gamma/delta T cell line but not in EBVtransformed B cells. I-309 is a chemoattractant for monocytes, but not for neutrophils. IP-10 is an approximately 8.5 kD polypeptide of 73 amino acids. The precursor form of human I-309 consists of 96 amino acids. To generate the mature I-309, the precursor cleaves its hydrophobic signal peptide. Human I-309 is thought to be a homologue of the mouse TCA3, and shows 42 % amino acid homology to mouse TCA3.Synonyms: C-C motif chemokine 1, CCL-1, SCYA1, Small-inducible cytokine A1, T lymphocyte-secreted protein I-309

    基因ID

    6346

    NCBI登录号

    NP_002972

    UniProt

    P22362
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