CCL1 抗体 (Biotin)
Quick Overview for CCL1 抗体 (Biotin) (ABIN116045)
抗原
See all CCL1 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Recognizes Human I-309
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交叉反应 （详细）
- Species reactivity (tested):Human.
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纯化方法
- Affinity Chromatography.
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免疫原
- Highly pure (>98%) recombinant Human I-309. Remarks: Molecular Weight: ~150 kDa
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anti-Chemokine (C-C Motif) Ligand 1 (CCL1) (AA 1-96) antibody
CCL1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 1 (CCL1) (AA 24-92) antibodyCCL1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 1 (CCL1) (C-Term) antibodyCCL1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 1 (CCL1) (AA 51-96) antibodyCCL1 适用: 人 WB, ELISA, ICC, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 1 (CCL1) (AA 24-96) antibodyCCL1 适用: 人 ELISA 宿主: 小鼠 Monoclonal 4E4 unconjugated
anti-Chemokine (C-C Motif) Ligand 1 (CCL1) antibodyCCL1 适用: Streptomyces hydrogenans WB 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 1 (CCL1) (AA 24-96) antibodyCCL1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- Direct ELISA: To detect Human I-309 by Direct ELISA (using 100 μL/well) a concentration ofapprox. 1.0 μg/mL of this antibody is required. This Biotinylated polyclonal antibody allows thedetection of at least 0.2-0.4 ng/well of recombinant Human I-309. Sandwich ELISA: To detect Human I-309 by Sandwich ELISA (using 100 μL/well) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This Biotinylated polyclonalantibody, in conjunction with anti-Human I-309 (PP1017P1 or PP1017P2) as a Captureantibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human I-309. Western Blot: To detect Human I-309 by Western Blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant Human I-309 is 1.5-3.0 ng/lane, under either reducingor non-reducing conditions.
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限制
- 仅限研究用
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溶解方式
- Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2 without preservatives.
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- CCL1 (Chemokine (C-C Motif) Ligand 1 (CCL1))
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别名
- CCL1 / I-309
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背景
- I-309 is a member of the C-C, or beta chemokine class. Other chemokines in this group include MIP1 alpha, MIP1 beta, RANTES, MCP1/2/3/4, mouse JE, 1 - 2 μg/mL antibody detects 20 and mouse MARC, eotaxin, HCC1 and C10. These chemokines act primarily as chemoattractants and activate monocytes, dendritic cells, T lymphocytes, natural killer cells, B lymphocytes, basophils and eosinophils. I-309 was originally identified by subtractive hybridization as a transcript that was present in a gamma/delta T cell line but not in EBVtransformed B cells. I-309 is a chemoattractant for monocytes, but not for neutrophils. IP-10 is an approximately 8.5 kD polypeptide of 73 amino acids. The precursor form of human I-309 consists of 96 amino acids. To generate the mature I-309, the precursor cleaves its hydrophobic signal peptide. Human I-309 is thought to be a homologue of the mouse TCA3, and shows 42 % amino acid homology to mouse TCA3.Synonyms: C-C motif chemokine 1, CCL-1, SCYA1, Small-inducible cytokine A1, T lymphocyte-secreted protein I-309
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基因ID
- 6346
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NCBI登录号
- NP_002972
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UniProt
- P22362
抗原
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