CCL14 抗体
Quick Overview for CCL14 抗体 (ABIN116039)
抗原
See all CCL14 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody reacts with Human Hemofiltrate CC Chemokine 1 (HCC-1).
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纯化方法
- Affinity chromatography.
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免疫原
- Highly pure (>98%) recombinant hHCC-1.
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anti-Chemokine (C-C Motif) Ligand 14 (CCL14) (AA 20-93) antibody
CCL14 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1F12 unconjugated
anti-Chemokine (C-C Motif) Ligand 14 (CCL14) (AA 20-93) antibodyCCL14 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 14 (CCL14) (AA 1-93) antibodyCCL14 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 14 (CCL14) (AA 20-93) antibodyCCL14 适用: 人 ELISA 宿主: 小鼠 Monoclonal 3B12 unconjugated
anti-Chemokine (C-C Motif) Ligand 14 (CCL14) (AA 20-93) antibodyCCL14 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Neutralization. ELISA: To detect hHCC-1 by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2-0.4 ng/well of recombinant hHCC-1. Western Blot: To detect hHCC-1 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hHCC-1 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2 without preservatives.
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- CCL14 (Chemokine (C-C Motif) Ligand 14 (CCL14))
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别名
- NCC2 / CCL14
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背景
- The CC cytokines are secreted proteins characterized by two adjacent cysteines. The cytokine encoded by this gene induces changes in intracellular calcium concentration and enzyme release in monocytes. This gene expresses both monocistronic and bicistronic transcripts. Bicistronic transcripts include the upstream cytokine gene CCL15. In addition, CCL14 undergoes alternative splicing in the bicistronic transcripts, it is unknown if its monocistronic transcript is also subject to alternative splicing.Synonyms: C-C motif chemokine 14, Chemokine CC-1/CC-3, HCC-1/HCC-3, NCC-2, SCYA14, Small-inducible cytokine A14
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分子量
- 150 kDa
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基因ID
- 6358
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NCBI登录号
- NP_116738
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UniProt
- Q16627
抗原
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