FGF2 抗体
Quick Overview for FGF2 抗体 (ABIN116009)
抗原
See all FGF2 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Human Fibroblast Growth Factor basic (FGF-2).
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纯化方法
- Affinity chromatography.
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免疫原
- recombinant human FGF-2 (hFGF-2)
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anti-Fibroblast Growth Factor 2 (Basic) (FGF2) (AA 143-250) antibody
FGF2 适用: 人, 小鼠, 大鼠, 兔, 绵羊 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Fibroblast Growth Factor 2 (Basic) (FGF2) (AA 159-288) antibodyFGF2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fibroblast Growth Factor 2 (Basic) (FGF2) antibodyKO Validated FGF2 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Fibroblast Growth Factor 2 (Basic) (FGF2) (Middle Region) antibodyFGF2 适用: 人, 小鼠, 大鼠, 兔, 犬, 绵羊, Cow, 豚鼠, 斑马鱼, 马 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Fibroblast Growth Factor 2 (Basic) (FGF2) (AA 26-153) antibody (APC)FGF2 适用: 兔 WB, IHC, IP, ICC 宿主: 豚鼠 Polyclonal APC
anti-Fibroblast Growth Factor 2 (Basic) (FGF2) (C-Term) antibodyFGF2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Fibroblast Growth Factor 2 (Basic) (FGF2) (AA 189-288) antibodyFGF2 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 1A9B2 unconjugated
anti-Fibroblast Growth Factor 2 (Basic) (FGF2) (AA 143-288) antibodyFGF2 适用: 人 ELISA, ICC 宿主: 小鼠 Monoclonal 1D6B5 unconjugated
anti-Fibroblast Growth Factor 2 (Basic) (FGF2) (AA 143-288) antibodyFGF2 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Fibroblast Growth Factor 2 (Basic) (FGF2) antibodyFGF2 适用: 人 WB 宿主: 兔 Polyclonal RB52659 unconjugated
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应用备注
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Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhFGF-2 (0.3 ng/mL), a concentration of 0.25-0.40 μg/mL of this antibody is required. ELISA: (Indirect): To detect hFGF-2 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hFGF-2. (Sandwich): To detect hFGF-2 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Human FGF-2 (PP1007B1, PP1007B2) as adetection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hFGF-2. Western Blot: To detect hFGF-2 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hFGF-2 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2 without preservatives.
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储存液
- Without preservative
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注意事项
- Centrifuge vial prior to opening!
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储存条件
- 4 °C
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储存方法
- Store the antibody at 2-8 °C. Do not freeze!
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- FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))
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别名
- FGF Basic / FGF2
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背景
- FGF basic (FGF2) is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. FGF2 is involved in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. FGF2 mRNA contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.Synonyms: BFGF, FGFB, Fibroblast growth factor 2 (basic), HBGF-2, HBGF2, Heparin-binding growth factor 2
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基因ID
- 2247
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NCBI登录号
- NP_001997
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UniProt
- P09038
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途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus, S100 Proteins
抗原
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