FGF1 抗体
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北京 101111
Quick Overview for FGF1 抗体 (ABIN116005)
抗原
See all FGF1 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Human Fibroblast Growth Factor acidic (hFGF-1)
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纯化方法
- Affinity chromatography.
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免疫原
- Highly pure (>98%) recombinant hFGF-acidic.
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应用备注
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Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhFGF-acidic (30 ng/mL), a concentration of 0.46 - 0.9 μg/mL of this antibody is required. ELISA: To detect hFGF-acidic by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2 - 0.4 ng/well of recombinant hFGF-acidic. Western Blot: To detect hFGF-acidic by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hFGF-acidic is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2 without preservatives.
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储存液
- Without preservative
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注意事项
- Centrifuge vial prior to opening!
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储存条件
- 4 °C
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- FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))
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别名
- FGF Acidic / FGF1
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背景
- The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these two growth factors.Synonyms: Acidic fibroblast growth factor, Beta-endothelial cell growth factor, ECGF-beta, FGFA, HBGF-1, HBGF1, Heparin-binding growth factor 1
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基因ID
- 2246
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NCBI登录号
- NP_000791
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UniProt
- P05230
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途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
抗原
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