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CCL3 抗体 (Biotin)

This Biotin-conjugated 兔 多克隆 anti-CCL3 antibody specifically detects CCL3 in WB 和 EIA. The antibody is reactive with 大鼠 samples.
产品编号 ABIN115967
发货至: 中国
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中国
北京 101111
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Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for CCL3 抗体 (Biotin) (ABIN115967)

抗原

See all CCL3 抗体
CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

适用

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大鼠

宿主

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克隆类型

  • 68
  • 37
多克隆

标记

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This CCL3 antibody is conjugated to Biotin

应用范围

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Western Blotting (WB), Enzyme Immunoassay (EIA)
  • 特异性

    Recognises Rat MIP-1 alpha.

    纯化方法

    Affinity Chromatography employing an immobilized Rat MIP-1-alpha matrix

    免疫原

    Highly pure (>98%) recombinant Rat MIP-1-alpha (Rat MIP-1-alpha).
  • 应用备注

    ELISA: To detect rat MIP-1-alpha by direct ELISA (using 100 l/well antibody solution) thisantibody can be used at a concentration of 0.15-0.30 μg/mL. Used in conjunction withcompatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinantrat MIP-1-alpha. Western Blot: To detect rat MIP-1-alpha by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant rat MIP-1-alpha is 1.5 - 3.0 ng/lane, under eitherreducing or non-reducing conditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 溶解方式

    Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

    缓冲液

    PBS, pH 7.2 without preservatives

    储存液

    Without preservative

    注意事项

    Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

    储存条件

    4 °C/-20 °C

    储存方法

    Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.
  • 抗原

    CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

    别名

    MIP1 alpha / CCL3

    背景

    Macrophage Inflammatory Protein 1 alpha (MIP1 alpha) and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximately 77 % and 70 % amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. Has adhesive effects on lymphocytes. MIP1 alpha can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. A signal transducing receptor, designated the C-C chemokine receptor-1 (C-C CKR-1) with seven transmembrane domains that bind MIP1 alpha, MIP1 beta, MCP1 and RANTES with varying affinities, has been isolated.Synonyms: C-C motif chemokine 3, CCL-3, G0/G1 switch regulatory protein 19-1, G0S19-1 protein, LD78 alpha, MIP-1-alpha, MIP1A, Macrophage Inflammatory Protein 1 alpha, SCYA3, Small-inducible cytokine A3, Tonsillar lymphocyte LD78 alpha protein

    基因ID

    25542

    NCBI登录号

    NP_037157

    UniProt

    P50229

    途径

    Cellular Response to Molecule of Bacterial Origin, Autophagy
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