CCL2 抗体 (Biotin)
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Quick Overview for CCL2 抗体 (Biotin) (ABIN115963)
抗原
See all CCL2 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Affinity chromatography.
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免疫原
- Highly pure (>98%) recombinant rat MCP-1(MCAF) (rat MCP-1(MCAF).
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应用备注
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ELISA: To detect rat MCP-1(MCAF) by direct ELISA (using 100 ml/well antibody solution) thisantibody can be used at a concentration of 0.15-0.30 μg/mL. Used in conjunction withcompatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinantrat MCP-1(MCAF). Western Blot: To detect rat MCP-1(MCAF) by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant rat MCP-1(MCAF) is 1.5-3.0 ng/lane, under eitherreducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2 without preservatives.
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))
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别名
- MCP1 / CCL2
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背景
- This chemotactic factor attracts monocytes and basophils but not neutrophils or eosinophils and augments monocyte anti-tumor activity. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. It may be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.Synonyms: C-C motif chemokine 2, HC-11, HC11, MCAF, MCP-1, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, SCYA2, Small-inducible cytokine A2
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基因ID
- 9606
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UniProt
- P13500
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途径
- Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
抗原
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