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IL-7 抗体 (Biotin)

This anti-IL-7 antibody is a 兔 多克隆 antibody detecting IL-7 in WB 和 EIA. Suitable for 小鼠.
产品编号 ABIN115887
发货至: 中国

Quick Overview for IL-7 抗体 (Biotin) (ABIN115887)

抗原

See all IL-7 (IL7) 抗体
IL-7 (IL7) (Interleukin 7 (IL7))

适用

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小鼠

宿主

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克隆类型

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多克隆

标记

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This IL-7 antibody is conjugated to Biotin

应用范围

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Western Blotting (WB), Enzyme Immunoassay (EIA)
  • 特异性

    This antibody reacts with Mouse Interleukin-7 (IL-7). Other species not tested.

    纯化方法

    Affinity Chromatography

    免疫原

    Highly pure (>98%) E.ocli derived recombinant Mouse IL-7 (Cat.-No PA014).
  • 应用备注

    ELISA: To detect mIL-7 by direct ELISA (using 100 l/well antibody solution) this antibody can beused at a concentration of 0.15-0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant mIL-7. Western Blot: To detect mIL-7 by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant mIL-7 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 溶解方式

    Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

    缓冲液

    PBS, pH 7.2 without preservatives

    储存液

    Without preservative

    注意事项

    Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
  • 抗原

    IL-7 (IL7) (Interleukin 7 (IL7))

    别名

    Interleukin-7 / IL7

    背景

    Interleukin 7 (IL7) is a lymphoid cell growth factor that affects pre-B, pro-B, and early T cells. IL7 was previously known as pre-B cell growth factor and lymphopoietin 1. IL7 supports the growth of early B cells from long-term lymphoid bone marrow cultures. It is mitogenic to thymocytes and enhances the response of cells to other stimuli such as polyhydroxyalkanoate (PHA) and concanavalin A (ConA). IL7 stimulates the proliferation of CD4+/CD8+ cells. The proliferative response of thymocytes to IL7 is not affected by antibodies to the T cell growth factors such as IL2, IL4 and IL6, suggesting that IL7 is capable of stimulating T cell proliferation through a pathway independent of the known T cell growth factors. Mature T cells respond to IL7 and Con A, but not to IL7 alone. In myeloid lineage cells, IL7 upregulates the production of pro-inflammatory cytokines and stimulates the tumoricidal activity of monocytes/macrophages. IL7 is expressed by adherent stromal cells from various tissues.Synonyms: IL-7

    基因ID

    16196

    NCBI登录号

    NP_032397

    UniProt

    P10168

    途径

    JAK/STAT Signaling
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