IL-7 抗体 (Biotin)
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北京 101111
Quick Overview for IL-7 抗体 (Biotin) (ABIN115887)
抗原
See all IL-7 (IL7) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody reacts with Mouse Interleukin-7 (IL-7). Other species not tested.
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纯化方法
- Affinity Chromatography
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免疫原
- Highly pure (>98%) E.ocli derived recombinant Mouse IL-7 (Cat.-No PA014).
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应用备注
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ELISA: To detect mIL-7 by direct ELISA (using 100 l/well antibody solution) this antibody can beused at a concentration of 0.15-0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant mIL-7. Western Blot: To detect mIL-7 by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant mIL-7 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2 without preservatives
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- IL-7 (IL7) (Interleukin 7 (IL7))
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别名
- Interleukin-7 / IL7
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背景
- Interleukin 7 (IL7) is a lymphoid cell growth factor that affects pre-B, pro-B, and early T cells. IL7 was previously known as pre-B cell growth factor and lymphopoietin 1. IL7 supports the growth of early B cells from long-term lymphoid bone marrow cultures. It is mitogenic to thymocytes and enhances the response of cells to other stimuli such as polyhydroxyalkanoate (PHA) and concanavalin A (ConA). IL7 stimulates the proliferation of CD4+/CD8+ cells. The proliferative response of thymocytes to IL7 is not affected by antibodies to the T cell growth factors such as IL2, IL4 and IL6, suggesting that IL7 is capable of stimulating T cell proliferation through a pathway independent of the known T cell growth factors. Mature T cells respond to IL7 and Con A, but not to IL7 alone. In myeloid lineage cells, IL7 upregulates the production of pro-inflammatory cytokines and stimulates the tumoricidal activity of monocytes/macrophages. IL7 is expressed by adherent stromal cells from various tissues.Synonyms: IL-7
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基因ID
- 16196
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NCBI登录号
- NP_032397
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UniProt
- P10168
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途径
- JAK/STAT Signaling
抗原
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