IL-10 抗体 (Biotin)
Quick Overview for IL-10 抗体 (Biotin) (ABIN115867)
抗原
See all IL-10 (IL10) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Recognizes Murine IL-10.
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交叉反应 （详细）
- Species reactivity (tested):Mouse.
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纯化方法
- Affinity Chromatography.
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免疫原
- Highly pure (>98%) recombinant Mouse IL-10.
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anti-Interleukin 10 (IL10) (AA 101-178) antibody
IL10 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 10 (IL10) (AA 19-178) antibodyIL10 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 10 (IL10) (AA 19-178) antibodyIL10 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 10 (IL10) antibodyIL10 适用: 人, 小鼠, 大鼠, Cow, 兔, Pig, 马, 犬, 猴, 小鸡, 山羊, 绵羊, 豚鼠, Avian, Cat, 驴, Hamster WB 宿主: 山羊 Polyclonal unconjugated
anti-Interleukin 10 (IL10) antibodyMicroarray verified IL10 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB971550 unconjugated
anti-Interleukin 10 (IL10) antibodyMicroarray verified IL10 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB312926 unconjugated
anti-Interleukin 10 (IL10) antibodyIL10 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
anti-Interleukin 10 (IL10) (AA 27-53) antibodyIL10 适用: 人 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 10 (IL10) (AA 1-178) antibodyIL10 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 10 (IL10) (AA 1-178) antibodyIL10 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C5 unconjugated
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应用备注
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ELISA: To detect mIL-10 by direct ELISA (using 100 ml/well antibody solution) this antibody can beused at a concentration of 0.15-0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant mIL-10. Western Blot: To detect mIL-10 by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 mg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant mIL-10 is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2 without preservatives.
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing. Centrifuge vial prior to opening.
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储存条件
- 4 °C/-20 °C
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储存方法
- The lyophilized antibody can be stored for one year from despatch at -20 °C. Reconstituted antibody is stable for two weeks at 2-8 °C, frozen aliquots are stable for six months at -20 °C.
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有效期
- 12 months
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- IL-10 (IL10) (Interleukin 10 (IL10))
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别名
- Interleukin-10 / IL10
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背景
- Interleukin 10 (IL-10) is a 178 amino acid protein that is primarily secreted by TH2 clones. IL-10 has dual functions, the first of which is the suppression of cytokine production by TH1 clones responding to antigen presented by monocyte and macrophage antigen presenting cells (APCs). The second function consists of the inhibition of response of cytokine targeted cells, possibly by the downregulation of CD25 (the interleukin-2 receptor) on macrophages and B lymphocytes. IL-10 exerts its biological activity through the IL-10 receptor (IL-10R), a 560 amino acid, 110 kDa glycoprotein whose expression can be induced in cultured macrophages and fibroblasts by elevated in HIV-1 infected individuals and has been implicated in the progression of the disease.Synonyms: CSIF, Cytokine synthesis inhibitory factor, IL-10, TGIF
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基因ID
- 16153
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NCBI登录号
- NP_034678
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UniProt
- P18893
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途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints
抗原
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