ELISA: In a sandwich ELISA (assuming 100 ?l/well), a concentration of 2 μg/mL of thisantibody will detect at least 0.2 ng/well of recombinant human FGF-basic when used withbiotinylated antigen affinity purified anti-human FGF-basic as the detection antibody at aconcentration of 1 μg/mL. Western Blot: To detect hFGF-basic by Western Blot analysis this antibody can be used at aconcentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hFGF-basic is 1.0-2.0 ng/lane, under non-reducingconditions. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Restore in sterile water to a concentration of 1.0 mg/mL.
缓冲液
PBS without preservatives.
储存液
Without preservative
储存条件
4 °C
储存方法
Store the antibody at 2-8 °C. DO NOT FREEZE! Avoid prolonged exposure to light.
FGF basic (FGF2) is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. FGF2 is involved in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. FGF2 mRNA contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.Synonyms: BFGF, FGFB, Fibroblast growth factor 2 (basic), HBGF-2, HBGF2, Heparin-binding growth factor 2