CCL3 抗体
Quick Overview for CCL3 抗体 (ABIN115769)
抗原
See all CCL3 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Recognizes Human Macrophage Inflammatory Protein 1 alpha (MIP1-alpha). Other species not tested.
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纯化方法
- Affinity Chromatography on Protein G
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免疫原
- Highly purified recombinant Human MIP-1 alpha.
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亚型
- IgG1
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anti-Chemokine (C-C Motif) Ligand 3 (CCL3) antibody
CCL3 适用: 大鼠, 小鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 3 (CCL3) (AA 24-92) antibodyCCL3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4E7 unconjugated
anti-Chemokine (C-C Motif) Ligand 3 (CCL3) (AA 24-92) antibodyCCL3 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 3 (CCL3) (AA 25-81) antibodyCCL3 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 3 (CCL3) (AA 24-92) antibodyCCL3 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 3 (CCL3) (AA 24-92) antibodyCCL3 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 3 (CCL3) (AA 59-87), (C-Term) antibodyCCL3 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB21614 unconjugated
anti-Chemokine (C-C Motif) Ligand 3 (CCL3) (AA 24-92) antibodyCCL3 适用: 大鼠 ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 3 (CCL3) (AA 24-92) antibodyCCL3 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 3 (CCL3) (AA 24-92) antibodyCCL3 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
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应用备注
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Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofHuman MIP1-alpha (100 ng/mL), a concentration of 5.0-10.0 μg/mL of this antibody isrequired. ELISA: In a Sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL ofthis antibody will detect at least 2000 pg/mL of recombinant Human MIP1-alpha when usedwith Biotin anti-Human MIP1-alpha antibody (cat. PP1050B) as the detection antibody at aconcentration of approximately 1.0-2.0 μg/mL. Western Blot: To detect HUman MIP1-alpha by Western Blot analysis this antibody can beused at a concentration of 2.0-4.0 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant Human MIP1-alpha is 1.0-2.0 ng/lane, undernon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore in sterile water to a concentration of 1.0 mg/mL.
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缓冲液
- PBS without preservatives
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.
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- CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))
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别名
- MIP1 alpha / CCL3
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背景
- Macrophage Inflammatory Protein 1 alpha (MIP1 alpha) and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximately 77 % and 70 % amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. Has adhesive effects on lymphocytes. MIP1 alpha can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. A signal transducing receptor, designated the C-C chemokine receptor-1 (C-C CKR-1) with seven transmembrane domains that bind MIP1 alpha, MIP1 beta, MCP1 and RANTES with varying affinities, has been isolated.Synonyms: C-C motif chemokine 3, CCL-3, G0/G1 switch regulatory protein 19-1, G0S19-1 protein, LD78 alpha, MIP-1-alpha, MIP1A, Macrophage Inflammatory Protein 1 alpha, SCYA3, Small-inducible cytokine A3, Tonsillar lymphocyte LD78 alpha protein
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基因ID
- 6348
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NCBI登录号
- NP_002974
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UniProt
- P10147
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途径
- Cellular Response to Molecule of Bacterial Origin, Autophagy
抗原
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