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IL-7 抗体

IL7 适用: 人 WB, EIA, Func 宿主: 小鼠 Monoclonal unconjugated
产品编号 ABIN115765
发货至: 中国
  • 抗原 See all IL-7 (IL7) 抗体
    IL-7 (IL7) (Interleukin 7 (IL7))
    适用
    • 48
    • 31
    • 26
    • 1
    • 1
    • 1
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    宿主
    • 64
    • 12
    • 11
    • 1
    • 1
    小鼠
    克隆类型
    • 67
    • 22
    单克隆
    标记
    • 48
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    • 4
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    • 2
    • 2
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    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
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    This IL-7 antibody is un-conjugated
    应用范围
    • 71
    • 38
    • 19
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    • 13
    • 7
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    Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)
    特异性
    This antibody recognises Human IL-7.
    纯化方法
    Affinity Chromatography on Protein A.
    免疫原
    Recombinant human IL-7.
    亚型
    IgG1
    Top Product
    Discover our top product IL7 Primary Antibody
  • 应用备注
    Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhIL-7 (0.5 ng/mL), a concentration of 0.05-0.08 μg/mL of this antibody is required. ELISA: In a Sandwich ELISA (assuming 100μL/well), a concentration of 2.0-4.0 μg/mL of thisantibody will detect at least 1000 pg/mL of recombinant Human IL-7 when used withBuiotin anti-Human IL-7 antibody (PP1029B1 or PP1029B2) as the detection antibody at aconcentration of approximately 0.5-1.0 μg/mL. Western Blot: To detect Hunman IL-7 by Western Blot analysis this antibody can be used ata concentration of 1.0-2.0 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant Human IL-7 is 2.0-4.0 ng/lane, under reducingconditions and 0.25-0.50 ng/lane, under non-reducing conditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 溶解方式
    Restore in sterile water to a concentration of 1.0 mg/mL.
    缓冲液
    PBS without preservatives.
    储存液
    Without preservative
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C/-20 °C
    储存方法
    Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
  • 抗原
    IL-7 (IL7) (Interleukin 7 (IL7))
    别名
    Interleukin-7 / IL7 (IL7 产品)
    别名
    IL7 antibody, IL-7 antibody, A630026I06Rik antibody, Il-7 antibody, hlb368 antibody, interleukin 7 antibody, IL7 antibody, Il7 antibody
    背景
    Interleukin 7 (IL7) is a lymphoid cell growth factor that affects pre-B, pro-B, and early T cells. IL7 was previously known as pre-B cell growth factor and lymphopoietin 1. IL7 supports the growth of early B cells from long-term lymphoid bone marrow cultures. It is mitogenic to thymocytes and enhances the response of cells to other stimuli such as polyhydroxyalkanoate (PHA) and concanavalin A (ConA). IL7 stimulates the proliferation of CD4+/CD8+ cells. The proliferative response of thymocytes to IL7 is not affected by antibodies to the T cell growth factors such as IL2, IL4 and IL6, suggesting that IL7 is capable of stimulating T cell proliferation through a pathway independent of the known T cell growth factors. Mature T cells respond to IL7 and Con A, but not to IL7 alone. In myeloid lineage cells, IL7 upregulates the production of pro-inflammatory cytokines and stimulates the tumoricidal activity of monocytes/macrophages. IL7 is expressed by adherent stromal cells from various tissues.Synonyms: IL-7
    基因ID
    3574
    NCBI登录号
    NP_000871
    UniProt
    P13232
    途径
    JAK/STAT Signaling
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