Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
特异性
The antibody will detect human VEGFR-3/FLT-4. In Western blots, this antibody shows a moderate cross-reactivity with VEGFR-2/KDR and a weak cross-reactivity with VEGFR-1/Flt-1.
纯化方法
Protein A Affinity Chromatography.
免疫原
Recombinant human soluble FLT-4 protein (110 kDa).
ELISA: 0.5-1.5 μg/mL, allows the detection of 0.25- 0.5 ng/well rhVEGFR-3/FLT-4. Western Blot: 0.5-1 μg/mL, it will detect approximately 5 ng/lane of rh VEGFR-3/FLT-4 underreducing conditions depending on the visualisation method. Immunoprecipitation: 1-5 μg/mL lysate or reaction volume. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Restore in sterile water/PBS to a concentration of > 0.5 mg/mL.
缓冲液
PBS, pH 7.4 containing no preservatives or stabilizers.
储存液
Without preservative
注意事项
This product is photosensitive and should be protected from light
储存条件
4 °C
储存方法
Store the antibody undiluted at 2-8 °C. DO NOT FREEZE.