ELISA: 1-15 μg/mL. Western blotting: 1-2 μg/mL. Flow cytometry and cell sorting (FACS): 2-5 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
缓冲液
PBS, pH 7.4, without preservatives
储存液
Without preservative
注意事项
Avoid repeated freeze-thaw cycles!
储存条件
4 °C/-20 °C
储存方法
The lyophilized antibody, thought stable at room temperature, is best stored desiccated below 0 °C. Reconstituted anti-TIE-2/tek is stable at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
TIE2 (tyrosine kinase with Ig and EGF homology domains 2) is expressed almost exclusively in endothelial cells in mice, rats and humans. This receptor possesses a unique extracellular domain containing two immunoglobulin like loops separated by three epidermal growth factor like repeats that are connected to three fibronectin type III like repeats. The ligand for the receptor is Angiopoietin 1. Defects in TIE2 are associated with inherited venous malformations, the TIE2 signaling pathway appears to be critical for endothelial cell smooth muscle cell communication in venous morphogenesis.Synonyms: Angiopoietin-1 receptor, TIE-2, TIE2, Tunica interna endothelial cell kinase, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE-2, p140 TEK