Immunoprecipitation. Western Blot. Immunohistochemistry on paraffin embedded sections: diluted of 1: 50-1: 100 in an ABC method (30 minutes at room temperature)Recommended Positive Control: Breast carcinoma Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
状态
Liquid
缓冲液
10 mM citrate buffer, pH 6.0 - containing sodium azide as preservative.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
HCDC47 is a member of MCM family, which allows DNA to replicate once per cell cycle. Expression of hCDC47 protein has been found in the nuclei of proliferative components of normal lymph nodes, bone marrow, epidermis and mucosa. Malignant tumors from several organs contain more nuclear hCDC47-positive cells than their normal counterparts.Synonyms: CDC47, CDC47 homolog, DNA replication licensing factor MCM7, MCM2, P1.1-MCM3, minichromosome maintenance complex component 7