KRT15 抗体 (C-Term)
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Quick Overview for KRT15 抗体 (C-Term) (ABIN114890)
抗原
See all KRT15 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- C-Term
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特异性
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Cytokeratin 15 is a type I cytokeratin without a defined type II partner.
Cellular Localization: Cytoplasmic. -
纯化方法
- Purified
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免疫原
- A 17-mer synthetic peptide from the C-terminus of human cytokeratin 15
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亚型
- IgG2a
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应用备注
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Immunoflourescence. Immunohistochemistry on Paraffin Embedded Sections: 1/25-1/75 dilution in an ABCmethod (30 minutes at room temperature). Requires high temperature antigen unmaskingwith 10 mM citrate buffer, pH 6.0 prior to immunostaining. Recommended Positive Control: Skin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- KRT15 (Keratin 15 (KRT15))
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别名
- Cytokeratin 15
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背景
- Cytokeratin 15 is expressed primarily in the basal keratinocytes of stratified tissues, including the fetal epidermis and fetal nail. A number of human cytokeratins are expressed during the development of stratified epithelia from one-layered polar epithelia and continue to be expressed in several adult epithelial tissues. Cytokeratin 15 (CK15) is a type I keratin without a defined type II partner whose expression in epidermal diseases has not been investigated. A monoclonal antibody raised against the last 17 amino acids of the CK15 polypeptide show that CK-15 is expressed primarily in the basal keratinocytes of stratified tissues, including the fetal epidermis and fetal nail. Although CK-15 in normal hair follicles was virtually absent from hair bulbs, it was expressed by a subset of keratinocytes in the outer root sheath. In human conjunctival epithelium, strong expression of CK-15 was observed in basal cells, whereas CK19 was expressed in both basal and suprabasal layers. Although the expression of K15 and K19 differ in humans and mice, specific staining patterns can be used to characterize the epithelial phenotype in normal and diseased ocular surface.Synonyms: CK-15, Cytokeratin-15, K15, KRT15, KRTB, Keratin, Keratin 15, Keratin-15, Type I Cytoskeletal 15
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基因ID
- 3866
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NCBI登录号
- NP_002266
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UniProt
- P19012
抗原
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