Clusterin 抗体
(5 references)Quick Overview for Clusterin 抗体 (ABIN114700)
抗原
See all Clusterin (CLU) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- The anti-Clusterin Antibody, Clone Hs-3 is a Mouse monoclonal antibody against Human Clusterin. Does not react with Cat, Dog, Bovine.
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纯化方法
- Affinity chromatography on protein A followed by DEAE-chromatography
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免疫原
- The antibody was prepared against Triton X-100 human sperm extract.
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亚型
- IgG1
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anti-Clusterin (CLU) (AA 331-449) antibody
CLU 适用: 人, 大鼠, 小鼠 ELISA, IHC (p), FACS, IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Clusterin (CLU) (AA 229-446) antibodyCLU 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Clusterin (CLU) (AA 227-448) antibodyCLU 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Clusterin (CLU) (AA 228-449) antibodyCLU 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Clusterin (CLU) (AA 226-447) antibodyCLU 适用: 兔 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C10 unconjugated
anti-Clusterin (CLU) (C-Term) antibodyVerified CLU 适用: 人 WB, IHC, ELISA, IF 宿主: 山羊 Polyclonal unconjugated
anti-Clusterin (CLU) (AA 23-120) antibodyCLU 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Clusterin (CLU) (AA 227-447) antibodyCLU 适用: 大鼠 WB, IHC 宿主: 小鼠 Monoclonal C1 unconjugated
anti-Clusterin (CLU) (C-Term) antibodyVerified CLU 适用: 大鼠, 小鼠 WB, ELISA, IF 宿主: 山羊 Polyclonal unconjugated
anti-Clusterin (CLU) (AA 402-501) antibodyCLU 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1A11 unconjugated
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应用备注
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ELISA. Immunocytochemistry. Immunofluorescence. Immunohistochemistry. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Add 0.1 mL of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.
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浓度
- 1.0 mg/mL
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缓冲液
- 0.05 M Phosphate buffer, 0.5 M NaCl, pH 7.2, 15 mM Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at -20 °C for 6 month.
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有效期
- 6 months
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: "Host-integration of a tissue-engineered airway patch: two-year follow-up in a single patient." in: Tissue engineering. Part A, Vol. 21, Issue 3-4, pp. 573-9, (2015) (PubMed).
: "Genotype-correlated expression of lysyl oxidase-like 1 in ocular tissues of patients with pseudoexfoliation syndrome/glaucoma and normal patients." in: The American journal of pathology, Vol. 173, Issue 6, pp. 1724-35, (2008) (PubMed).
: "Alteration in the activation state of new inflammation-associated targets by phospholipase A2-activating protein (PLAA)." in: Cellular signalling, Vol. 20, Issue 5, pp. 844-61, (2008) (PubMed).
: "Lymphatic reprogramming of microvascular endothelial cells by CEA-related cell adhesion molecule-1 via interaction with VEGFR-3 and Prox1." in: Blood, Vol. 110, Issue 13, pp. 4223-33, (2007) (PubMed).
: "Impact of cryopreservation on extracellular matrix structures of heart valve leaflets." in: The Annals of thoracic surgery, Vol. 81, Issue 3, pp. 918-26, (2006) (PubMed).
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: "Host-integration of a tissue-engineered airway patch: two-year follow-up in a single patient." in: Tissue engineering. Part A, Vol. 21, Issue 3-4, pp. 573-9, (2015) (PubMed).
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- Clusterin (CLU)
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别名
- Apolipoprotein J (Apo J)
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背景
- Clusterin is a 75-80 kD disulfide-linked heterodimeric protein containing about 30 % of N-linked carbohydrate rich in sialic acid, but truncated forms targeted to the nucleus have also been identified. The precursor polypeptide chain is cleaved proteolytically to remove the 22-mer secretory signal peptide and subsequently between residues 227/228 to generate the alpha and beta chains. These are assembled antiparallel to give a heterodimeric molecule in which the cysteine-rich centers are linked by five disulfide bridges and are flanked by two predicted coiled-coil alpha-helices and three predicted amphipathic alpha-helices. The six sites of N-linked glycosylation are indicated as yellow spots. Across a broad range of species clusterin shows 70 % to 80 % of sequence homology. It is ubiquitously expressed in most mammalian tissues and can be found in plasma, milk, urine, cerebrospinal fluid and semen. It is able to bind and form complexes with numerous partners such as immunoglobulins, lipids, heparin, bacteria, complement components, paraoxonase, beta amyloid, leptin and others. Clusterin has been ascribed a plethora of functions such as phagocyte recruitment, aggregation induction, complement attack prevention, apoptosis inhibition, membrane remodelling, lipid transport, hormone transport and/or scavenging, matrix metalloproteinase inhibition. A detailed mechanism of clusterin has not been defined. One tempting hypothesis says that clusterin is an extracellular chaperone protecting cells from stress induced by degraded and misfolded protein precipitates.Clusterin is up- or downregulated on the mRNA or protein level in many pathological and clinically relevant situations including cancer, organ regeneration, infection, Alzheimer disease, retinitis pigmentosa, myocardial infarction, renal tubular damage, autoimmunity and others.Synonyms: 40, 40, AAG4, APOJ, Aging-associated gene 4 protein, CLI, Clusterin, Complement cytolysis inhibitor, Complement-associated protein SP-40, KUB1, Ku70-binding protein 1, NA1/NA2, SP-40, Testosterone-repressed prostate message 2
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基因ID
- 1191
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NCBI登录号
- NP_976084
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UniProt
- P10909
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途径
- Apoptosis, Negative Regulation of intrinsic apoptotic Signaling
抗原
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